Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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2022 - 20263

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Fecha de inicio: 2020Tema: search.filters.subject.LECHUGA

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  • Miniatura
    Item type:Ítem,
    Determinación de los subtipos de Toxoplasma gondii en muestras de lechuga (Lactuca sativa l.) del mercado América de Ambato
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2026-01)
    Pacheco Jiménez, Yesenia Camila
    ;
    De La Torre Olvera, Helena Maritza
    Toxoplasma gondii is a parasite that causes ocular toxoplasmosis, a zoonosis that affects one-third of the world's population and significantly harms pregnant women and immunocompromised individuals. It has been shown to have several routes of transmission, mainly related to cat feces and undercooked meat. However, there are also routes of transmission through raw vegetables, especially green leafy vegetables such as lettuce (Lactuca sativa L). In Ecuador, there is no information on the presence of this parasite in vegetable matrices, nor on its molecular characterization. The objective of this research was to determine the presence of T. gondii in lettuce sold in the América market in Ambato and to identify whether it belongs to subtype II or III by amplifying the SAG2 gene and using nested PCR. A descriptive and exploratory analysis was carried out, in which 21 samples from seven market stalls were analyzed. Eleven of these samples were identified microscopically, and seven of them showed molecular evidence of the presence of T. gondii (33, percent). However, none of the samples were compatible with subtypes II or III, suggesting that non-archetypal strains of T. gondii may be circulating in the city. In conclusion, the study determined the presence of T. gondii in lettuce samples and provides preliminary evidence on the genetic variability of the parasite, laying the groundwork for future research.
  • Miniatura
    Item type:Ítem,
    Elaboración de platos biodegradables germinables a partir de materiales agroindustriales
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02)
    Lozada Pérez, Daisy Paola
    ;
    Moreno Toasa, Gabriel Alejandro
    In this research, germinable biodegradable plates were developed from agro-industrial materials to avoid the consumption of disposable single-use plates, which are thrown into landfills, seas and oceans causing damage to the marine flora and fauna. Undoubtedly, since the plates have a germinative character, they become a unique and innovative proposal for the consumer because after its useful life is over, the plate returns to the earth and has the capacity to complete the biodegradation and germination process. As for the methodology, the physicochemical, mechanical and optical properties of germinable biodegradable plates were identified using an experimental design and the best treatment was determined, resulting in 25 percent corn (Zea Mays); 30 percent second-use Kraft paper (without ink, not bleached). In addition, after the statistical analysis it was determined that the seeds of edible species radish (Raphanus sativus) and lettuce (Lactuca sativa), do not have significant differences that affect the analysis of the properties. Finally, the plates biodegraded in 45 days while, the germination percentage for radish was 85 percent and lettuce 75 percent. It was demonstrated that the natural fibers of the germinable biodegradable dishes were ideal, and the dishes are an ecological alternative to reduce environmental pollution.
  • Miniatura
    Item type:Ítem,
    Determinación de la concentración de metales pesados (cadmio, plomo y cromo) en la lechuga (Lactuca sativa) que se cultiva en la parroquia Presidente Urbina del cantón Píllaro
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería en Alimentos, 2022-03)
    Lasluiza Quishpe, María Fernanda
    ;
    Cerda Mejía, Liliana Alexandra
    Lettuce is a vegetable frequently used in the diet due to its low caloric value and is rich in vitamins and minerals. Environmental contamination has increased in recent years because of anthropogenic activities, being the most interesting the irrigation water and soil where they are grown because they have presented heavy metals such as cadmium, lead, and chromium, which are considered toxic and therefore it affects the health of the population when they ingest it through contaminated food with high concentrations. This research determined the presence of cadmium, lead, and chromium in samples composed of lettuce, irrigation water, and agricultural soil collected in the Presidente Urbina parish of the Pillaro canton belonging to the province of Tungurahua, through the atomic absorption spectrophotometry method. The three samples were compared with national and international standards, as a result, it was found that the analyzed samples have concentrations below the maximum permissible limit for cadmium, lead, and chromium. Therefore, the crops of the Presidente Urbina parish are free of heavy metal contaminants, this is guaranteeing the health of these consumers.