Carrera de Alimentos

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34799

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Evaluación del efecto de la enzima Cel6D de Paenibacillus barcinonensis en la degradación de residuos agroindustriales
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Alimentos, 2025-02) Zurita Gancino, Joaquín Fernando; Cerda Mejía, Liliana Alexandra
The accumulation of agro-industrial residues generated by agricultural activity in Ecuador represents a significant environmental challenge because inadequate management can cause contamination and affect public health. The use of specific enzymes for cellulose degradation has been widely studied. Thus, this study focused on evaluating the effect of the enzyme Cel6D from Paenibacillus barcinonensis on the degradation of these residues, seeking a biotechnological alternative for their use and reduction of environmental impact. The methodology employed included the construction of a vector for the expression of the enzyme in Escherichia coli, followed by purification by affinity chromatography. The enzymatic activity of Cel6D was evaluated on various swollen and non-swollen agro-industrial substrates. The results showed that the enzyme presented enhanced enzymatic activity on swollen substrates, highlighting a yield of 1.84 49 milliunits of cellulose activity per milligram on cocoa husk and 1.49 49 milliunits of cellulose activity per milligram on rice bran, suggesting that swelling facilitates the access of the enzyme to cellulose. It was concluded that Cel6D is effective in the degradation of agro-industrial wastes, especially when the substrates are treated by acid swelling to increase its accessibility. This research highlights the importance of optimizing the swelling and purification processes to maximize enzyme activity and its potential application in biorefineries, thus contributing to a circular and sustainable economy in waste management in Ecuador.
Evaluación del efecto de la enzima Xyn30D de Paenibacillus barcinonensis en la degradación de residuos agroindustriales
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Alimentos, 2024-08) Burbano Palacios, David Fernando; Cerda Mejía, Liliana Alexandra
The extraction and processing of raw materials in the wood, paper, and agricultural industries are responsible for greenhouse gas emissions and the destruction of biodiversity. In response, the use of renewable and earth-abundant raw materials, such as lignocellulose—a xylan-rich component—has been sought. However, the complex lignocellulosic structure presents a challenge to physical and chemical methods of degradation, leading to the proposed use of enzymes for this purpose. The bacterium Paenibacillus barcinonensis is known for its ability to produce extracellular enzymes that can break down biomass, including xylanases of the GH30 family. Among these enzymes, xylanase Xyn30D has demonstrated efficiency in lignocellulose degradation. In this project, we analyzed the activity of xylanase Xyn30D, from plasmid construction to expression in E. coli BL21 (DE3) and purification by immobilized metal affinity chromatography (IMAC). Furthermore, we evaluated its activity in four different agro-industrial wastes with high xylan content, which have a significant impact on the agricultural industry in Ecuador and worldwide. The substrates sugarcane bagasse and corn stover showed positive enzyme activity, while cocoa husk and rice husk did not exhibit significant values in terms of xylose units per gram of enzyme. This lack of activity is attributed to the presence of inhibitors in the chemical composition of the latter substrates.