Carrera de Biotecnología

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    Caracterización nanoscópica superficial de películas de almidón extraído de tubérculos andinos
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) De La Vega Camalle, Jhomara Mercedes; Casado Rojo, Santiago
    Biopolymers are an alternative to synthetic polymers. In particular, starch-based films have a huge research potentiality: they are non-toxic, easily degradable, use green chemistry, and can be obtained from many different natural sources. In order to use them for practical applications it’s important to understand the ultimate causes of their useful macroscopic properties, and they may be found at the nanoscopic scale. Here we present analysis performed by atomic force microscopy, characterizing the nanoscopic topographic surfaces of samples extracted from the Andean tubers melloco (Ullucus tuberosus), mashua (Tropaeolum tuberosum), oca (Oxalis tuberosa) and potato (Solanum tuberosum). Measurements revealed significant differences among the films extracted from these tubers. These results were contrasted to macroscopic measurements such as moisture content, total soluble matter, elastic properties, opacity and IR absorption spectra. Phase contrast segregation and topography AFM tapping images permitted the correlation. Hence, we could relate those samples with granular and phase segregations at the nanoscale to the less elastic forces and water affinity.
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    Determinación de componentes fenólicos y antioxidantes en harina de camote morado (Ipomeas batatas) y mashua (Tropaeolum tuberosum)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) Chamba Maza, Jonnatan Franklin; Pérez Aldas, Lander Vinicio
    The lack of awareness about the consumption of functional and nutritious foods has generated multiple problems in health, being considered a negative factor that has been increasing over the years. For this reason and with the purpose of providing a contribution to society around health and human nutrition, this research project aimed to provide relevant information on the Andean crops of Ecuador, such as sweet potato and mashua, toexpose its composition in terms of its phenolic content and antioxidant capacity. This information can be exploited in a way that supports the development of nutritional foods that allow achieving a better quality of life by reducing nutritional health problems. First, the HPLCUV Visibletechnique was performed on flour samples for the identification of phenolic compounds using gallic acid as standard, determining compounds such as p-coumaric acid, cyanidin, caffeic acid and derivatives, tyrosol glycoside and catechin. The antioxidant capacity was determined by the DPPH technique, obtaining a percentage of 60.976 percent for sweet potato and 69.974 percent for mashua, which are considered significant, finding the mashua flour with a higher antioxidant capacity.