Carrera de Biotecnología

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800

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    Análisis de la actividad antiinflamatoria y agregación plaquetaria compuestos bioactivos del extracto etanólico de Cedrón (Aloysia citrodora paláu)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Valencia Armas, Joselin Gabriela; Pérez Aldás, Lander Vinicio
    Currently, access to large medical treatments is a global challenge, which drives a renewed interest in traditional medicine and medicinal plants, however, the lack of scientific studies limits their acceptance in public health. This research analyzes the bioactive compounds of the ethanolic extract of cedron (Aloysia citrodora Paláu) for its anti-inflammatory and antiplatelet aggregation activity. The cedron plant, also known as verbena, confers a range of therapeutic uses including the reduction of inflammation and protection against oxidative stress. Wherein, gallic acid reduces inflammation, while quercetin exhibits anticoagulant properties. The extraction yield was 17.01 percent, and the content of total phenols and flavonoids was found to decrease as the extract was diluted, with maximum values of 163.45 milligram (mg) over gram (g) and 99.12 mg over g, respectively, at the highest concentration. In terms of anti-inflammatory activity, the cedron extract showed an inhibition of albumin denaturation of 61.85 percent, lower than diclofenac (95.1 percent), but comparable as it is diluted. In addition, the extract inhibited platelet aggregation similarly to the control drug at all concentrations tested. The flavonoids and phenols present in the ethanolic extract of cedron exhibit significant anti-inflammatory and antiplatelet aggregation activity, standing out as a natural alternative. These findings contribute to the development of new organic products and to the growth of the Flor Andina company.
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    Evaluación de la actividad antioxidante e hipoglucemiante in vitro de la combinación de extractos etanólicos de Marco (Ambrosia arborescens), Quishuar (Buddleja incana) y Cedrón (Aloysia citrodora)
    (2024-02) Jaque Pavón, Alison Paola; Pérez Salinas, Ruth Narcisa
    Diabetes Mellitus (DM) is a chronic disease characterized by the high amount of glucose present in the blood, causing hyperglycemia in the patient. One of the factors involved in the progressive development and complications of the disease is the cellular damage produced by the excess of free radicals present in the body. Plants present a great variety of biological properties, conferred by the compounds they possess, such as antioxidant and hypoglycemic activity, given mainly by phenols and flavonoids present in the plant matrix. Therefore, these molecules are studied as alternatives in the treatment of hyperglycemia. This study evaluates the antioxidant and hypoglycemic activity of four combinations of ethanolic extracts of Marco, Quishuar and Cedron. Phenols and flavonoids were quantified using the Folin-Ciocalteu method and the aluminum chloride method. Then, antioxidant and hypoglycemic activity was evaluated by the DPPH method and enzymatic assays with α-glucosidase and α-amylase enzymes. The combination with the highest biological activity was microencapsulated with maltodextrin and a combination of maltodextrin and gum arabic. The Marco-Quishuar (MQ) and Quishuar-Cedron (QC) combinations presented a higher content of the mentioned metabolites. The MQ combination presented a greater inhibition of the DPPH radical and of the α-glucosidase and α-amylase enzymes; therefore, this combination was microencapsulated. By means of infrared spectroscopy (FTIR), the efficiency of the microencapsulation was analyzed. Finally, the combination without microencapsulation exhibited better biological activity.
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    Evaluación de la actividad antioxidante de extractos de Aloysia citrodora (Cedrón) y Prunus serotina (Capulí) en Andamios biológicos
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Gavilanes Suárez, Jonathan André; Núñez Villacís, Lorena de los Ángeles
    Ischemic heart disease (IHD) is a disease caused by obstruction of blood flow, due to factors such as oxidative stress due to reactive oxygen species (ROS). In certain cases, tissue transplants are required, so tissue engineering (TE) proposes possible alternatives for their treatment, such as the application of biological scaffolds (BSs) with antioxidants. This study focused on the evaluation of the antioxidant activity of extracts of Aloysia citrodora (cedrón) and Prunus serotina (capulí) in BSs. Extracts were obtained by maceration and the content of phenols and flavonoids was quantified. By inhibiting DPPH, its antioxidant activity was calculated and a combination of extracts was made. The best combination of extracts was incorporated in the preparation of BSs. To check the antioxidant activity in BS, FT-IR was performed in addition to a DPPH test. Morphological and physical properties were also evaluated by SEM, mechanical tests and permeability. The yield percentages in obtaining extracts were 11.34 for lemon verbena and 12.32 for capulí. The combination of extracts in a 7:3 ratio for capulí:cedron presented an increase in the percentage of inhibition of free radicals compared to pure extracts. The addition of the extract in ABs increased the inhibition percentage to 70.17. Its incorporation into BSs allows the presence of ROS to be reduced. This shows that BS with extract has the potential to be applied in TE for the development of endothelial tissue and combat IHD.
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    Evaluación de la actividad antioxidante e hipoglucémica in vitro de extractos de Marco (Ambrosia arborescens), Quishuar (Buddleja incana), Cedrón (Aloysia citrodora) y Capulí (Prunus serotina)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Jara Irigoya, Jonathan David; Tubón Usca, Irvin Ricardo
    Diabetes mellitus is a chronic disease that affects a long part of de world population. The main symptom of diabetes is high blood glucose levels (hyperglycemia), which triggers an imbalance in the body, producing secondary pathologies associated with oxidative stress generated by this metabolic dissorder. This research project aimed to evaluate the antioxidant and hypoglycemic capacity of Marco, Quishuar, Cedrón and Capulí leaves. The extracts were obtained through maceration and infusion, yielding between 10.3 percent and 18.30 percent, the phytochemical profile of each plant species was characterized through qualitative tests to determine the presence or absence of metabolites such as, alkaloids, reducing carbohydrates, coumarins, phenols, triterpenes, flavonoids, saponins, resins, free ammonium acids, and antocyanins. Quantitative determinations of total phenols and flavonoid content were also conducted. The antioxidant capacity was evaluated using the free radical scavenging assay with 2,2-diphenyl-1-picrylhydrazil (DPPH), resulting in the percentages of free radical inhibition between 65.40 percent and 84.61 percent. Furthermore, the alpha-amylase inhibition assay was performed to determine de hypoglycemic capacity, with inhibition percentages ranging from 19.35 percent to 48.95 percent. This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.
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    Evaluación de la actividad antioxidante e hipoglucémica in vitro de extractos de Marco (Ambrosia arborescens), Quishuar (Buddleja incana), Cedrón (Aloysia citrodora) y Capulí (Prunus serotina)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Cunalata Lascano, Erick Bladimir; Tubón Usca, Irvin Ricardo
    Diabetes mellitus is a chronic disease that affects a long part of de world population. The main symptom of diabetes is high blood glucose levels (hyperglycemia), which triggers an imbalance in the body, producing secondary pathologies associated with oxidative stress generated by this metabolic dissorder. This research project aimed to evaluate the antioxidant and hypoglycemic capacity of Marco, Quishuar, Cedrón and Capulí leaves. The extracts were obtained through maceration and infusion, yielding between 10.3 percent and 18.30 percent, the phytochemical profile of each plant species was characterized through qualitative tests to determine the presence or absence of metabolites such as, alkaloids, reducing carbohydrates, coumarins, phenols, triterpenes, flavonoids, saponins, resins, free ammonium acids, and antocyanins. Quantitative determinations of total phenols and flavonoid content were also conducted. The antioxidant capacity was evaluated using the free radical scavenging assay with 2,2-diphenyl-1-picrylhydrazil (DPPH), resulting in the percentages of free radical inhibition between 65.40 percent and 84.61 percent. Furthermore, the alpha-amylase inhibition assay was performed to determine de hypoglycemic capacity, with inhibition percentages ranging from 19.35 percent to 48.95 percent. This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.