Carrera de Biotecnología

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Author: search.filters.author.Jerez Lluglla, Gabriela EstefaniaSubject: search.filters.subject.ESCHERICHIA COLI

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    Evaluación de la presencia de genes de resistencia a carbapenémicos, tigeciclina y colistina mediada por plásmidos en aislados de Escherichia coli procedentes del río Cutuchi y del canal Latacunga-Salcedo-Ambato
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Jerez Lluglla, Gabriela Estefania; Calero Cáceres, William Ricardo
    The Cutuchi River and the Latacunga-Salcedo-Ambato irrigation channel exhibits significant contamination by antibiotic-resistant bacteria from wastewater discharges. This study analyzed E. coli isolates collected from April 2022 to March 2023 in both water bodies, focusing on the presence of last resort antibiotic resistance genes (carbapenems, tigecycline, colistin) transmitted by plasmids (LARGs). Under the research line of Microbiology and Biotechnology, the presence of LARGs in E. coli isolates was evaluated using conventional Polymerase Chain Reaction (PCR) and gel electrophoresis. Transconjugation tests were performed using the E. coli J53 as recipient strain. Phenotypic resistance profiles were determined by the Kirby-Bauer method and broth microdilution, and the genotypic profile was evaluated using conventional PCR, ERIC-PCR, and gel electrophoresis. Three isolates with mcr-1 colistin mobile resistance genes were detected, two carriers of carbapenem-hydrolyzing betalactamase SHV, and one carrier of carbapenemase OXA-48, which represents the first environmental isolation reported in Ecuador. Transconjugation determined the transmissibility of these genes by horizontal gene transfer (HGT). Phenotypic analysis revealed that the isolates carrying the evaluated genes are extremely drug-resistant (XDR). In addition, genotypic analysis determined that most of the studied genes are transferable by transconjugation and are associated with other antibiotic resistance genes.