Carrera de Biotecnología

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    Evaluación de la actividad antioxidante e hipoglucemiante in vitro de la combinación de extractos etanólicos de Marco (Ambrosia arborescens), Quishuar (Buddleja incana) y Cedrón (Aloysia citrodora)
    (2024-02) Jaque Pavón, Alison Paola; Pérez Salinas, Ruth Narcisa
    Diabetes Mellitus (DM) is a chronic disease characterized by the high amount of glucose present in the blood, causing hyperglycemia in the patient. One of the factors involved in the progressive development and complications of the disease is the cellular damage produced by the excess of free radicals present in the body. Plants present a great variety of biological properties, conferred by the compounds they possess, such as antioxidant and hypoglycemic activity, given mainly by phenols and flavonoids present in the plant matrix. Therefore, these molecules are studied as alternatives in the treatment of hyperglycemia. This study evaluates the antioxidant and hypoglycemic activity of four combinations of ethanolic extracts of Marco, Quishuar and Cedron. Phenols and flavonoids were quantified using the Folin-Ciocalteu method and the aluminum chloride method. Then, antioxidant and hypoglycemic activity was evaluated by the DPPH method and enzymatic assays with α-glucosidase and α-amylase enzymes. The combination with the highest biological activity was microencapsulated with maltodextrin and a combination of maltodextrin and gum arabic. The Marco-Quishuar (MQ) and Quishuar-Cedron (QC) combinations presented a higher content of the mentioned metabolites. The MQ combination presented a greater inhibition of the DPPH radical and of the α-glucosidase and α-amylase enzymes; therefore, this combination was microencapsulated. By means of infrared spectroscopy (FTIR), the efficiency of the microencapsulation was analyzed. Finally, the combination without microencapsulation exhibited better biological activity.
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    Evaluación de la actividad antioxidante e hipoglucémica in vitro de extractos de Marco (Ambrosia arborescens), Quishuar (Buddleja incana), Cedrón (Aloysia citrodora) y Capulí (Prunus serotina)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Cunalata Lascano, Erick Bladimir; Tubón Usca, Irvin Ricardo
    Diabetes mellitus is a chronic disease that affects a long part of de world population. The main symptom of diabetes is high blood glucose levels (hyperglycemia), which triggers an imbalance in the body, producing secondary pathologies associated with oxidative stress generated by this metabolic dissorder. This research project aimed to evaluate the antioxidant and hypoglycemic capacity of Marco, Quishuar, Cedrón and Capulí leaves. The extracts were obtained through maceration and infusion, yielding between 10.3 percent and 18.30 percent, the phytochemical profile of each plant species was characterized through qualitative tests to determine the presence or absence of metabolites such as, alkaloids, reducing carbohydrates, coumarins, phenols, triterpenes, flavonoids, saponins, resins, free ammonium acids, and antocyanins. Quantitative determinations of total phenols and flavonoid content were also conducted. The antioxidant capacity was evaluated using the free radical scavenging assay with 2,2-diphenyl-1-picrylhydrazil (DPPH), resulting in the percentages of free radical inhibition between 65.40 percent and 84.61 percent. Furthermore, the alpha-amylase inhibition assay was performed to determine de hypoglycemic capacity, with inhibition percentages ranging from 19.35 percent to 48.95 percent. This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.
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    Evaluación de la actividad antioxidante e hipoglucémica in vitro de extractos de Marco (Ambrosia arborescens), Quishuar (Buddleja incana), Cedrón (Aloysia citrodora) y Capulí (Prunus serotina)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Jara Irigoya, Jonathan David; Tubón Usca, Irvin Ricardo
    Diabetes mellitus is a chronic disease that affects a long part of de world population. The main symptom of diabetes is high blood glucose levels (hyperglycemia), which triggers an imbalance in the body, producing secondary pathologies associated with oxidative stress generated by this metabolic dissorder. This research project aimed to evaluate the antioxidant and hypoglycemic capacity of Marco, Quishuar, Cedrón and Capulí leaves. The extracts were obtained through maceration and infusion, yielding between 10.3 percent and 18.30 percent, the phytochemical profile of each plant species was characterized through qualitative tests to determine the presence or absence of metabolites such as, alkaloids, reducing carbohydrates, coumarins, phenols, triterpenes, flavonoids, saponins, resins, free ammonium acids, and antocyanins. Quantitative determinations of total phenols and flavonoid content were also conducted. The antioxidant capacity was evaluated using the free radical scavenging assay with 2,2-diphenyl-1-picrylhydrazil (DPPH), resulting in the percentages of free radical inhibition between 65.40 percent and 84.61 percent. Furthermore, the alpha-amylase inhibition assay was performed to determine de hypoglycemic capacity, with inhibition percentages ranging from 19.35 percent to 48.95 percent. This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.This study results can serve as basis for future research focused on using medicinal plants to create pharmaceutical formulations as an alternative treatment for hyperglycemia caused by diabetes.