Tesis Medicina Veterinaria

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Subject: search.filters.subject.ANTIBIÓTICOS

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    Evaluación de la sensibilidad a los antibióticos y presencia de genes de resistencia en Lactococcus garvieae aislado en carne de pollo que se expende en Ambato
    (2024-08) Chávez Romero, Anderson Xavier; Cruz Quintana, Sandra Margarita
    Lactococcus garvieae is a Gram-positive pathogenic bacterium, considered an emerging zoonotic pathogen, as reports of human infection are increasing. In the present research work, the antibioresistance of the L. garvieae strain and the presence of resistance genes in L. garvieae isolated from chicken meat sold in Ambato were evaluated. Sensitivity was identified using the Kirby Bauer method for the following antibiotics: Linezolid, Ceftriaxone, Amoxicillin+clavulanic acid, Lincomycin and Oxytetracycline. After obtaining results where four of the five antibiotics were resistant: Linezolid, Ceftriaxone Lincomycin and Oxytetracycline where we chose oxytetracycline, which showed the highest resistance and is the most widely used antibiotic in livestock farming. An arduous investigation was carried out to identify resistance genes, tetD and tetA, then the DNA of L. garvieae was extracted by the method of Aljanabi & Martinez modified, the PCR technique was carried out with the corresponding primers of the genes and ended with an electrophoresis where it was not identified that it has tetD and tetA genes of resistance to oxytetracycline and finally it was investigated on the molecular mechanisms of resistance of L. garvieae to the different resistant antibiotics.
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    Evaluación de la presencia de los genes de resistencia a antibióticos betalactámicos y glucopéptidos en Mammaliicoccus sciuri aislado de la carne de pollo que se expende en Ambato
    (2024-08) Barreno Mayorga, Jessica Fernanda; Cruz Quintana, Sandra Margarita
    Antibiotic resistance represents a significant public health problem due to the ability of bacteria to cause difficult-to-treat infections. The objective of this research is to detect the presence of resistance genes to beta-lactam antibiotics (amoxicillin + clavulanic acid) and glycopeptides (vancomycin) in the bacteria Mammaliicoccus sciuri isolated from chicken meat sold in Ambato. The research was carried out by collecting Mamamlicocus samples isolated from chicken meat and analyzing them in the laboratory to determine the presence of the aforementioned resistance genes, using PCR, DNA extraction and electrophoresis. Obtaining as results the presence of resistance genes in beta-lactams with an amplification of the band of 162 bp but no amplification of glycopeptides was found, which means that there are different ways for the existence of resistance. The identification and monitoring of these genes are crucial to evaluate food safety and prevent the spread of resistant bacteria in the final food chain.
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    Evaluación de la sensibilidad a los antibióticos y factores de virulencia de bacterias Gram negativas aisladas de la carne de pollo que se expenden en el cantón de Ambato (Comamonas kerstersii, Aeromonas veronii, Pseudomonas aeruginosa)
    (2024-02) Ramírez Pérez, Juan José; Cruz Quintana, Sandra Margarita
    The following research work was developed in the Laboratories of the Technical University of Ambato, with the objective of evaluating the sensitivity to antibiotics and virulence factors of the following bacteria: Comamonas kerstersii, Aeromonas veronii, and Pseudomonas aeruginosa, isolated from meat of chicken that is spent in the Ambato canton. We worked with 6 samples, carrying out the management of the experiment in three phases. The statistical method used was the Kolmogorov Smirov method to determine if there is a normal distribution. Demonstrating the results, the following data: Pseudomonas aeruginosa and Comamonas kerstersii have greater sensitivity to the antibiotics used, while Aeromonas veronii showed resistance. The molecular mechanisms of resistance vary according to the type of bacteria and the antibiotic. Finally, as a defense mechanism, bacteria develop virulence factors to evade the response of the body's immune system. Keywords: Comamonas kerstersii, Aeromonas veronii, and Pseudomonas aeruginosa, sensitivity, resistance, antibiotic, bacteria, virulence factors.