Tesis Medicina Veterinaria

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Evaluación de la actividad antimicrobiana del aceite esencial microencapsulado de Hierba Luisa (Cymbopogon citratus) sobre bacterias Gram positivas aisladas de piel de conejo (Oryctolagus cuniculus)
(2024-02) Jaramillo Panimboza, Katherine Salomé; Cruz Quintana, Sandra Margarita
The objective of the research was to evaluate the antimicrobial activity of the microencapsulated essential oil of Lemongrass (Cymbopogon citratus) on Grampositive bacteria isolated from rabbit skin (Oryctolagus cuniculus), two Gram-positive bacteria (Paenibacillus profundus and Staphylococcus succinus) were molecularly identified using a 16s marker, the agar diffusion method in wells to measure antimicrobial activity, determined that the two bacterial strains did not form halos of inhibition, the minimum inhibitory concentration (MIC) in liquid medium was determined at 5% for the P. profundus strain with an optical density of 0.251, with respect to the S. succinus strain it was established that at 25% concentration of the oil there was bacterial growth at an optical density of 0.487, the colony forming units (CFU) for the S. succinus strain were determined at 15% of the oil concentration, with an average range of 2.00 CFU, being the best treatment, while for the P. profundus bacteria it was established from 15%, 20% and 25%, as it does not exist in any of them. the growth of UFC.