Tesis Medicina Veterinaria

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Evaluación de la presencia de los genes de resistencia a antibióticos betalactámicos y glucopéptidos en Mammaliicoccus sciuri aislado de la carne de pollo que se expende en Ambato
(2024-08) Barreno Mayorga, Jessica Fernanda; Cruz Quintana, Sandra Margarita
Antibiotic resistance represents a significant public health problem due to the ability of bacteria to cause difficult-to-treat infections. The objective of this research is to detect the presence of resistance genes to beta-lactam antibiotics (amoxicillin + clavulanic acid) and glycopeptides (vancomycin) in the bacteria Mammaliicoccus sciuri isolated from chicken meat sold in Ambato. The research was carried out by collecting Mamamlicocus samples isolated from chicken meat and analyzing them in the laboratory to determine the presence of the aforementioned resistance genes, using PCR, DNA extraction and electrophoresis. Obtaining as results the presence of resistance genes in beta-lactams with an amplification of the band of 162 bp but no amplification of glycopeptides was found, which means that there are different ways for the existence of resistance. The identification and monitoring of these genes are crucial to evaluate food safety and prevent the spread of resistant bacteria in the final food chain.