Tesis Medicina Veterinaria

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/36415

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Efecto antihelmíntico in vitro de extractos de arbustivas ricas en metabolitos secundarios sobre diferentes estadios del ciclo biológico de Haemonchus contortus
(2023-09) Rodríguez Calle, María Mercedes; Aragadvay Yungán, Ramón Gonzalo
The indiscriminate use of antiparasitic drugs contributes to resistance to them. Currently, natural alternatives have been sought such as the use of secondary metabolites as antiparasitics, such as tannins that negatively affect the nutrition, movement, and reproduction of gastrointestinal nematodes in animals. The objective of this work was to evaluate the in vitro anthelmintic effect of the hydroalcoholic extract of two tannin-rich shrubs, through its application on cultures of larvae and eggs of Haemoncus contortus. The extracts of Albizia lophantha and Acacia mearnsii were applied in 4 concentrations (T0: pure extract; T1: 0.04 ml/ml; T2: 0.08 ml/ml and T3: 0.12 ml/ml) 4 repetitions of each treatment were performed and the results obtained were analyzed with the ANOVA and Tukey test in the InfoStat 2020 version program. The inhibition of hatching was evaluated as an effectiveness variable. The most effective treatments with Albizia lophantha were: T0 with a 100% inhibition and T3 with a 33% inhibition at 24h, while with Acacia mearnsii the treatments that showed the highest inhibition were T0: 100% and T3: 29.5% at 24h. As for larval survival with the Albizia lophantha extract, the most effective treatments with 0% survival were T0 at 24h and T3 at 48h, while with the Acacia meanrsii extract the T0 and T3 treatments presented 0% survival at 24 and 48h respectively.
Efecto de la eCG en tratamientos super ovulatorios subsecuentes, sobre la fertilidad en la coneja y la viabilidad en los gazapos
(2023-09) Saigua Caicedo, Fernando David; Aragadvay Yungán, Ramón Gonzalo
Nowadays, the use of exogenous hormones is an increasingly preferred alternative by producers of all farm species, where through it they manage to obtain a greater number of benefits, versus using traditional production methods. In this investigation, the effect and use of equine Chorionic Gonadotropin (eCG) in superovulatory treatments was verified, on established parameters: such as fertility, through follicular and oocyte quality, serum E2 levels and receptivity to the male, in addition to measuring the viability of the rabbits resulting from this protocol through the number of pups per birth, mortality rate and daily weight gain. For this investigation, 27 large hybrid breed multiparous rabbits were considered, and 4 fertile male rabbits, which were treated under the same environmental and nutritional standard, the females were randomly distributed, in a random design, with three completely different treatments ( 9 females per treatment) and each treatment was repeated twice. The treatments consisted of: T0 (control 25UI for synchronization), T1 (medium dose 50UI for superovulation) and T2 (high dose 100UI for superovulation) these doses were administered intramuscularly. The use of eCG for superovulatory treatments in rabbits resulted in an increase in receptivity, ovulation rate and follicular growth, however, it was shown to show no differences in weight gain and number of pups per birth.
Efecto del plasma seminal y vitamina E sobre el proceso de crioconservación y calidad post descongelado de semen ovino
(2023-09) Inca Pilco, Dayana Mishel; Aragadvay Yungán, Ramón Gonzalo
In this research work, the effect of seminal plasma and vitamin E on the post-thaw quality of ovine semen was evaluated using three treatments: T0: control treatment where a commercial extender was used, T1: vitamin E (25%) was added to the commercial extender, T2: vitamin E (25%) plus seminal plasma (50%) was added to the commercial extender. We worked with 3 rams and 1 ewe, evaluating a total of 210 sheep straws. Using a completely randomized design with 7 replicates per treatment, the results were compared by Tukey's statistical test with a significance level of 95% using the statistical program Infostat. The following variables were analyzed in two phases: for fresh semen, an individual evaluation per ejaculate/carrier was performed, consisting of a macroscopic study (volume, color, translucency) and a microscopic evaluation using conventional techniques (concentration, mass motility, morphology, vitality). For the post-thawed semen, a pool of heterospermic semen was made and aliquots were formed for each corresponding treatment, diluted, packaged, frozen and the post-thawed semen was evaluated microscopically at 0 and 2 hours by conventional techniques (concentration, individual mobility, morphology, vitality) plus osmotic resistance (HOST) and acrosome integrity. In conclusion, positive results were obtained in post-thawed semen quality by adding vitamin E and seminal plasma to the commercial diluent.
Evaluación de semen porcino centrifugado de raza Pietrain para la obtención de dosis con alta concentración espermática
(2024-08) Cadena Cando, Jean Sebastián; Aragadvay Yungán, Ramón Gonzalo
The aim of this research was to evaluate the centrifuged porcine sperm of the Pietrain breed in order to obtain dose with high concentration of sperm, for which it was needed to work with 4 treatments: Tx0 (Control treatment) and different gravitational forces Tx1 (1200g), Tx2 (2200g) and Tx3 (3200g). 143 ml of pork sperm from Pietrain breed were applied, using a design completely random with 6 repetitions per treatment, the results were compared through the Tukey test to the 0.05, where different parameters were evaluated in order to determine the sperm quality. The variables considered to analyze the fresh sperm were obtained through a macroscopic research (color, smell, volume) and through microscopic tests (morphology, sperm concentration, mass and individual motility), meanwhile, for the treatments with centrifuged sperm the variables were added (alive and dead spermatozoids percentage in microscopic tests). The obtained results were statistically analyzed by ANOVA (Variance Analysis), where there was no meaningful difference found, except from the sperm concentration which demonstrated statistic differences in the treatments (Tx2) and (Tx3) showing that the sperm concentration varies depending the centrifuged force applied.
Evaluación del efecto del jugo de tuna (Opuntia ficus-indica l. mill) y vitamina E sobre los parámetros de calidad seminal en la crio preservación de semen ovino
(2023-03) Chango Muñoz, María de Los Ángeles; Aragadvay Yungán, Ramón Gonzalo
The present investigation was carried out with the objective of evaluating the effect of prickly pear juice (Opuntia ficus-indica L. Mill) and vitamin E on semen quality parameters in cryopreservation of ovine semen. For which three treatments were applied: Tc: the control treatment where a commercial diluent (Triladyl) was obtained, Tt: Tuna juice was obtained as a natural diluent and Tt+ VE: the diluent based on tuna juice was applied. and vitamin E was added. We worked with 3 rams and 1 female, semen was extracted three times a week and a pool was made with the three ejaculations, therefore we worked with heterospermic semen. A study with a random design was applied with a 3*3 factorial arrangement with a time factor at 0 - 2 -4 hours post-thawing. For the results in the comparison of means between the treatments, it was analyzed by means of an analysis of variance and contrasts with the Tukey test in the Infostat program, the differences were considered statistically significant at the level of P < 0.05. Semen was analyzed in two stages with corresponding variables, fresh semen volume, translucency, color was evaluated macroscopically and concentration, mobility, morphology, vitality was analyzed microscopically and in post-thawed semen at 0-2-4 hours concentration, mobility, morphology and vitality. Concluding that Tuna juice and vitamin E do influence seminal quality parameters.