Carrera Ingeniería Bioquímica
Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/809
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Item Efecto in vitro de Valeriana rígida y Valeriana Decussata en la apoptosis tardía en células cancerígenas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Lizano Guzmán, César Paúl; Vargas López, José Homero; Carrero Castillo, Yenddy NayghitCancer represents a public health problem worldwide, especially breast cancer, which occupies the first place of incidence in the country, followed by thyroid cancer. Ecuador has a very biodiverse territory with an extensive variety of medicinal plants that offer a possible therapeutic potential; however, there are few studies that validate these properties. Reason enough to contemplate a number of possibilities in the molecular and biochemical study of different types of plants, in particular of the valerian ones. The objective of this project was to evaluate the expression of the p53 gene by means of qRT-PCR of the Valeriana rigid and Decussata extracts in the MCF7 cell line, in addition to late apoptosis. The results showed that the relative expression of the pro-apoptotic gene p53 in relation to the control showed that the treatments; Valerian Decussata root and Decussata leaf stem produce increased expression of the p53 gene. On the contrary, for Valerian Rigida root and Rigida leaf stem showed a lower expression than that of the control. Regarding the late apoptosis tests using APO / bdru Tunel for control, MCF7 cancer cells were evidenced, with fragmented nuclei. For the treatment with Valeriana Decussata Root, cell fragments and other DNA fragments were observed that may show the end of the apoptotic pathway, as Valeriana Decussata leaf stem only one apoptotic cell was observed, while for rigid Valerian leaf stem and root no evidence of late apoptosis was observed.Item Actividad antioxidante y citotóxica in vitro de Valeriana decussata y Valeriana rígida en células cancerígenas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-03) Guacales Reinosa, Danny Alexander; Carrero Castillo, Yenddy Nayghit; Vargas López, José HomeroCancer represents a health problem worldwide, leaving with its high death rates that continue to rise. The treatments used to counteract the disease have high costs and are losing their effectiveness as cancer cells become increasingly resistant; hence the importance of looking for new therapeutic alternatives, in order to reestablish a new panorama in favor of the search for new phytocomposites extracted from plants, such as valerian species, characterized by its composition of terpenes, valeric acid, valtrates and essential oils with various anti-inflammatory, anticonvulsant and anti-tumor properties. The objective of the present investigation was the determination of the antioxidant and cytotoxic activity in vitro of extracts obtained from Valerian decussata and Valerian rigid in the breast cancer cell line (MCF-7). For this, cell viability studies were carried out using the MTT method, immunohistochemical tests, nitric oxide determination using the Griess method and the antioxidant activity with the TROLOX test. The presence of antioxidants and compounds with biological activity that acted were evidenced. beneficially over the MCF-7 cell line type.Item Capacidad proapoptótica in vitro de Valeriana rígida y Valeriana decussata en células cancerígenas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Williams Ibarra, Jeniffer Andrea; Carrero Castillo, Yenddy NayghitCancer represents a public health problem level world, with high incidence and mortality rates in developed and undeveloped countries. Currently there are different therapeutic treatments to eliminate or prevent the proliferation of cancer cells, however, the most are invasive, and they have side effects. Today there are natural therapeutic alternatives with the aim of develop more efficient and less invasive treatments to fight cancer. Faced with this problem, the research done it is based on in vitro evaluation of the proapoptotic and cytotoxic capacity of valerian extracts on the breast cancer cell line. Cell viability assays (MTT), immunohistochemistry assays were performed by fluorescence microscopy and quantitative reverse transcription PCR for gene expression of proteins. The results obtained showed the decrease in cell proliferation and variability in the gene expression of apoptotic proteins in breast cancer cells after being treated with optimal doses of Valeriana rigid and Valeriana decussata extracts.Item Capacidad antioxidante in vitro de Valeriana microphylla y Valeriana plantaginea en células cancerígenas(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-01) Moreta Pacha, Erika Jazmín; Vargas López, José Homero; Carrero Castillo, Yenndy NaygithFree radicals play an important role in cell metabolism, proving to be beneficial or deadly for the body. Due to their aggressive nature, these can contribute to the genesis of cancer; however, there are defense strategies using antioxidants. Plants play an important role within therapeutic resources, which are bioactive agents used in pharmaceutical laboratories in research and therapeutic proposals for various pathologies such as breast cancer. The objective of the research was based on determining the antioxidant capacity of Valeriana microphylla and Valeriana plantaginea extracts in MFC-7 (breast cancer) cells. The extracts were made in two groups, the first was the combination of Leaves Stems and the second roots for each species; For the determination of total solids, water was used as a solvent and for cell culture ethanol, with the following results: the total soluble solids with the best performance were obtained in Valeriana plantaginea Leaves Stems. The cytotoxic activity was quantified by the MTT method; where the extract of Leaves Stems of Valeriana plantaginea, reached the highest percentage. Through immunohistochemical staining, the expression of ROS, MITOSOX, GLUTTATION was evidenced using specific monoclonal antibodies for each of the proteins, which show significant differences compared to the control. The antioxidant capacity was determined using the Trolox technique, showing a low antioxidant capacity for both species of valerians. The results allowed us to infer the therapeutic potential of these valerian species, however, complementary studies should be carried out.Item Evaluación de la capacidad citotóxica y proapoptótica del látex de Brosimum utile (Sande) sobre células HeLa como posible alternativa terapéutica para el tratamiento del cáncer de cuello uterino(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2018-08) Acosta Ganán, Michael Andrés; Carrero Castillo, Yendy Nayghit; López Hernández, Orestes Darío