Browsing by Author "Pazmiño Orozco Andrés Sebastián"

Now showing 1 - 1 of 1

Presencia de genes de resistencia a los antibióticos en Shigella spp. aislada de carne de pollo que se expende en el cantón de Ambato.
(2024-11) Pazmiño Orozco Andrés Sebastián; Cruz Quintana Sandra Margarita
Shigella spp. is a common causal agent of diarrheal diseases around the world. The treatment of choice for shigellosis is the administration of antibiotics. However, the resistance that Shigella spp. has developed to these molecules poses a major challenge in the need to find effective treatment alternatives. Transmission of Shigella spp. is via the fecal-oral route, however, infection can result from the ingestion of contaminated food where poor hygiene during handling can be considered a risk factor for the occurrence of disease. Likewise, unhygienic conditions during the slaughter of animals could constitute one of the main sources of contamination of meat for human consumption. In this research, we start from previous studies where, from samples of chicken meat taken from points of sale and slaughterhouses in Ambato, 17 strains of Shigella spp. were isolated and molecularly identified and some of them, showed marked resitance mainly to two antibiotics: amoxicillin-clavulanate (AMC) and ciprofloxacin (CIP). The aim of this investigation was to verify the presence of a resistance gene for each of the antibiotics described above. For AMC, the blaTEM gene was searched for, which encodes for class A ESBL (extended spectrum β-lactamases), finding a prevalence of 23.53%, while for CIP, the gyrA gene was searched for, which encodes for the A subunits of DNA gyrase, finding a prevalence of 100%. One of the resistance mechanisms for AMC would be the production of TEM-like ESBLs, while specific mutations in a small region near the start of the gyrA gene, called the quinolone resistance determining region (QRDR), have been widely linked to CIP resistance in Enterobacteriaceae.