Browsing by Author "Culqui Arcos, Evelyn Johanna"

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    Evaluación de la actividad antioxidante y el tiempo de vida útil de un extracto obtenido a partir de la mora (Rubus glaucus)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Culqui Arcos, Evelyn Johanna; Fernández Rivero, Danae
    In recent years, interest innatural products has increased considerably, thanks to their therapeutic properties, especially their antioxidant activity. Among these products are extracts, which can be obtained from red fruits. However, to ensure their quality, it is crucial to determine their shelf life. An outstanding example of red fruits rich innatural antioxidants with phenolic compounds is the blackberry (Rubus glaucus). In this study, blackberry extracts were obtained using a two-factor experimental design, in which ethanol concentration and extraction time were evaluated. The phenolic content was determined using the Folin-Ciocalteu method, where the results identified as the best treatment the one that presented the highest phenolic content, reaching 62.594 milligram equivalents of gallic acid per gram of dry weight, under an ethanol concentration of 50 percent and an extraction time of 20 minutes. In addition, its antioxidant activity was evaluated using the DPPH method, obtaining an inhibition percentage of 90.544 percent. Similarly, a value of 161.003 equivalent micromoles of Trolox per gram of dry weight was recorded. Finally, the stability of the extract was analyzed for 30 days and a shelf life of 33.6540 days when stored in amber glass bottles was established using the Minitab statistical tool with a confidence level of 95 percent. Its behavior followed a first-order kinetic model, with a rate constant of 0.0031 days.