Evaluación de la sensibilidad a los antibióticos y presencia de genes de resistencia en Lactococcus garvieae aislado en carne de pollo que se expende en Ambato

Abstract

Lactococcus garvieae is a Gram-positive pathogenic bacterium, considered an emerging zoonotic pathogen, as reports of human infection are increasing. In the present research work, the antibioresistance of the L. garvieae strain and the presence of resistance genes in L. garvieae isolated from chicken meat sold in Ambato were evaluated. Sensitivity was identified using the Kirby Bauer method for the following antibiotics: Linezolid, Ceftriaxone, Amoxicillin+clavulanic acid, Lincomycin and Oxytetracycline. After obtaining results where four of the five antibiotics were resistant: Linezolid, Ceftriaxone Lincomycin and Oxytetracycline where we chose oxytetracycline, which showed the highest resistance and is the most widely used antibiotic in livestock farming. An arduous investigation was carried out to identify resistance genes, tetD and tetA, then the DNA of L. garvieae was extracted by the method of Aljanabi & Martinez modified, the PCR technique was carried out with the corresponding primers of the genes and ended with an electrophoresis where it was not identified that it has tetD and tetA genes of resistance to oxytetracycline and finally it was investigated on the molecular mechanisms of resistance of L. garvieae to the different resistant antibiotics.