Análisis filogenético de Komagataeibacter xylinus K2G30=UMCC2756 para la producción de biofilms a partir de suero lácteo

Abstract

Bacterial cellulose, being an organic, renewable, and biodegradable biopolymer has received considerable attention. Here, the production of bacterial cellulose by Komagataeibacter xylinus K2G30 (UMCC 2756) was studied in static culture using Hestrin and Schramm (HS) medium with total replacement of glucose by whey and lactic acid. Furthermore, phylogenetic trees were made from sequences of the 16S ribosomal RNA gene from the species K. xylinus, K. europaeus, K. hansenii and K. intermedius, which revealed that they lacked the lac operon responsible for lactose hydrolysis. Due to these characteristics, they can explain that the highest production of BC by the K. xylinus K2G30 strain was achieved with the use of lactic acid as the sole carbon source. Results provide important information for future studies on the synthesis of BC from different carbon sources, including raw materials from industrial waste such as whey, which must be previously fermented for better assimilation.