Obtención de cepas probióticas a partir de diferentes sustratos de origen orgánico de la provincia de Santo Domingo de los Tsáchilas – Ecuador

Abstract

The study consists of obtaining probiotic strains from three samples of cassava, sugar cane and bovine feces, as an alternative to the use of antibiotics in the livestock sector and to counteract the spread of resistance genes (RAM), since probiotics are live microorganisms that have a positive effect on the health of the host. The 63 strains obtained from the three samples have the phenotypic characteristics of model probiotic microorganisms such as catalase negative, Gram positive, with creamy or white round morphology. Likewise, the probiotic potential of the strains was evaluated by in vitro tests such as cell adhesion, tolerance to gastrointestinal conditions such as low pH and bile salt concentrations. Also, the selection of probiotic strains should satisfy safety criteria, they should not be toxic or pathogenic, susceptible to antibiotics, without hemolytic activity and have antagonistic activity against pathogens. Finally, only two strains from the cassava and sugar cane samples have the best characteristics and best efficiency to be considered probiotic strains, because they did not present hemolytic activity and have cell adhesion, can survive at pH 2 to 3 and bile salts of 0.3 to 0.5 percent, had susceptibility to six antibiotics with the highest range of inhibition compared to other strains, however, no strain presented antagonistic activity against E. coli bacteria.