Eficacia antimicrobiana de aceites esenciales de tomillo (T. vulgaris), CANELA (C. verum), romero (R. officinalis), laurel (L. nobilis) sobre cepas de Pasteurella multocida Y Bordetella bronchiseptica

Abstract

The aim of this study was the evaluation of the antimicrobial activity of Thyme (T. vulgaris), Cinnamon (C. verum), Rosemary (R. officinalis), Laurel (L. nobilis) essential oils on certified strains of Pasteurella multocida ATCC® 12945 TM * and Bordetella bronchiseptica ATCC® 4617 TM *. The minimum inhibitory concentrations (MICs) were estimated by microdilution method; and the plate plating (Mueller Hinton agar) was applied for the estimation of the minimum bactericidal concentrations (MBCs). The MICs of the evaluated essential oils, at the experimental conditions were: P. multocida ATCC® 12945 TM * (L. nobilis 0.8%, C. verum 3.8%, T. vulgaris 2.4% and R. officinalis 10.5%); for B. bronchiseptica ATCC® 4617 TM *. (L. nobilis 1.8%, C. verum 6.7%, T. vulgaris 10.1% and R. officinalis 3.5%). The MBCs of the evaluated essential oils were: L. nobilis 2.1%, C. verum 7%, T. vulgaris 10.3% and R. officinalis 7% for B. bronchiseptica ATCC® 4617 TM *; for P. multocida ATCC® 12945 TM * were L. nobilis 1%, C. verum 4.3%, T. vulgaris 2.7% and R. officinalis 11.1%. The inhibition percentages of the evaluated essential oils (compared to gentamicin 160 mg / mL) was found between 33.78% - 40.84% in the case of P. multocida and between 5.98%- 22.35% for B. bronchiseptica.