Evaluación de la estabilidad de un extracto con compuestos bioactivos presentes en la cáscara de papa (Solanum Tuberosum) provenientes de residuos de restaurantes de comidas rápidas ubicada en la parroquia Celiano Monge, provincia Tungurahua

Abstract

This research aims to evaluate the time of shelf life of the steroidal glycoalkaloid present in the peel extract of potato, determining in turn the percentage of participation of antioxidant activity and anti-inflammatory compared to the extract. Eight extracts were elaborated evaluating sun drying and dehydrator, plant material: solvent 1:20 and 1:40 and extraction time 5 and 10 minutes. The estimation of glycoalkaloid (alpha-solanine) content in potato peel was performed using an alpha-solanine standard curve. Through a statistical analysis it was determined that sample 8 (Drying by dehydrator, plant material: solvent 1:20 and extraction time of 10 minutes) obtained the highest amount of alpha-solanine (7, 42 mg of alpha-solanine over mL of extract). The extracts were analyzed by FTIR to verify the functional groups of the steroidal glycoalkaloid, where hydroxyl groups, the methyl and methylene groups (part of the steroidal ring), CN or CC bonds, stretching vibrations glycosidic were observed. The determination of shelf life, antioxidant activity and anti-inflammatory activity was performed with two batches (Ambient and Refrigerated), the samples stored under refrigeration presented higher stability within 30 days of analysis. Steroidal glycoacaloids presented minimal antioxidant activity (4 percent) and moderate anti-inflammatory activity (20 percent). Therefore, storing these compounds under refrigeration generates a longer shelf life and stability in biological activities.