Análisis in silico de las rutas metabólicas necesarias para la síntesis de celulosa bacteriana en Komagataeibacter xylinus a partir de lactosa

Abstract

Bacterial Cellulose symbolizes a multifaceted resource of exceptional capabilities and fascinatingproperties. Currently, the demand for this exopolysaccharide has progressively increasing, in function of the optimization of processes and the implementation of agro-industrial waste as alternative carbon sources. In such a context, the archetype productor (K. xylinus) represents one of the most valuable organisms in the biotechnological field, a scenario that has made it possible to demonstrate its enormous power to synthesize BC from a wide range of substrates. However, some authors have elucidated its inability to metabolize lactose, caused by the lack of genes responsible for the transport and catabolism of this substrate. Here, DSM 2004 and the analogous strain E25 (identified by phylogenetic analysis), were studied with the aim of identifying missing genes and minimal genetic equipment. A critical analysis of metabolic pathways in BAL and BAA was carried out to list the transporter and the enzymes involved in the process of interest. In this, the summarized proteins were evaluated according to kinetic parameters, where the best results report access code and both peptide and nucleotide sequence of the gene that encodes them. In this, the genes of Escherichia coli K-12 (Lactose permease), Alicyclobacillus acidocaldarius ATCC 27009 (β – galactosidase), Bifidobacterium longum subsp. Infantis ATCC 15697 (galactokinase), and Escherichia coli XL1-Blue (galactose-1-P-uridyltransferase) were selected. Finally, the modeling of the new biosynthetic route presented two related modules: i) Transport and hydrolysis of Lactose and ii) Biosynthesis of CB, after the total replacement of glucose by whey lactose.