Validación del método analítico por espectrofotometría uv-vis para la determinación de edulcorantes en bebidas azucaradas consumidos en la provincia de Tungurahua

Abstract

The present research focuses on the validation of the calibration curves of the UV Vis spectrophotometry analysis method for the detection of synthetic sweeteners of sucralose, aspartame and acesulfame K, the study is aimed at possible applications in sugary drinks. For the analysis, standard solutions were prepared with buffer and chromophore solutions in known concentrations. The study was carried out in a UV Vis Thermo Scientific Evolution 60S series spectrophotometer. The calibration curves were made with the proposed concentrations for each standard solution with which the value of R squared was determined, in all cases it was greater than 99 percent, in turn, the equations of calibration for each solution. A statistically significant linearity was determined for the process and a sensitivity of 0.0252; 0.0018 and 0.2332 for sucralose, aspartame and acesulfame K. The detection limit calculated for each standard solution corresponds to 0.0547, 0.0249 microgram per milliliter and 0.0459 milligram per milliliter for sucralose, aspartame, and acesulfame K, respectively; the calculated expanded uncertainty was between 7.44, 19.08 and 21.5 percent for sucralose, aspartame and acesulfame K, respectively. In turn, through a bibliographic review, it was possible to determine that HPLC chromatography is a method for detecting high precision synthetic sweeteners with very sensitive detection limits, however, spectrophotometry is also a good option for this type of analysis that presents a great sensitivity.