Evaluación de dos métodos de propagación de mortiño (Vaccinium floribundum Kunth), para la obtención de plántulas

Abstract

The paramo soil composite substrates with Raizal 400 showed the highest vigor of the cuttings (at the beginning of the trial), number of shoots (at 30, 45 and 60 days), shoot length (at 15, 30 and 45 days). The highest vigor at the base of the stake was observed in the composite treatment based on paramo soil plus Induktor without the presence of necrosis in the cuttings. In relation to the percentage of contamination of the first test, it was similar for both culture media in both the first and the second evaluation. Likewise, in the third evaluation, no differences were observed in the percentage of vigorous explants. The percentage of contamination of the second trial showed that the highest percentage of contamination in the first and third evaluation was reached in the culture flasks containing the MS (Murashige y skoog) 7.5 g sucrose, 0.5 g phytagel and 1.11 g MS for 250ml, culture medium. However, in the second evaluation, the highest percentage of contamination was detected in the AM the elements are half of the MS medium, except the phytagel: 3.75 g of sucrose, 0.5 g of phytagel and 0.555 g of MS medium for 250 ml. In the third evaluation, there were no differences in the percentage of contamination between the MS and AM culture media. With the present work, it was possible to establish plants under V. floribundum conditions by cutting which will help to form donor plant banks to continue with the future in vitro establishment phase of explants.