Obtención de un banco de plantas donantes de arándanos (Vaccinium corymbosum L.) para el establecimiento in vitro de segmentos nodales en medio de cultivo semisólido

Abstract

The present research was developed with the purpose of determining how certain factors influence both ex vitro rooting and in vitro establishment, among which we have the composition of the substrate, the type and concentration of auxins, the disinfection agent, the composition of MS salts, and illumination, among others. A completely randomized design was used. With regard to the substrate composition and auxin concentration and type, it was determined that there were no statistical differences between the treatments in terms of the percentage of establishment variable, and it was also determined that the nodal segments that were 3 mm thick and between 1 cm and 3 cm long were those that had a higher percentage of rooting, good results were obtained in the in vitro tests, among them we have the concentration of the disinfecting agent (NaClO), thus achieving a lower percentage of contamination by using the disinfecting agent (NaClO) at a concentration of 3%. As for the influence of the type and concentration of auxins on the in vitro establishment of blueberries, statistical differences were observed among the treatments analyzed for each of the response variables. Finally, the factors of MS medium composition, illumination conditions, type of culture flask and Phytagel concentration did not show significant differences among the treatments evaluated for the variable percentage of contamination and vigor of the nodal segments