Microencapsulación del probiótico Saccharomyces cerevisiae var. boulardii mediante secado por aspersión con polímeros Eudragit®

Abstract

Saccharomyces cereviseae var. boularii is a non-pathogenic, non-systemic yeast, resists acidic pH, its optimum growth temperature is 37. It is considered a probiotic as it generates enzymes capable of degrading toxins produced by pathogens within the human intestine. Microencapsulation was performed by spray drying the yeast with Eudragit® enteric coating polymers (L-100, S-100, L-10055) using a mini–Spray Dryer (BUCHI-B290) at an inlet air temperature 120 Celsius degrees and an outlet air temperature of 70 Celsius degrees. The yield of the process was calculated and by means of statistical analysis it was determined that the most suitable polymer to microencapsulate this yeast was L-100 with a value of 53,41 percent. To analyze the viability of the microencapsulates, the plate diffusion technique was performed, by means of which it was determined that the most viable polymer was L-100 with a concentration of 60:40 (polymer: yeast) and whose value was equal to 2.5 E 10 CFU for each gram of microencapsulate. Hard capsules of Saccharomyces cereviseae var. boularii with its respective analysis of release of the active principle, by means of which it was determined that the best formulation contained: 53 percent of yeast microencapsulates, 1 percent of sodium starch glycolate, 1 percent of colloidal silicon dioxide and 20 percent of maltodextrin microgranulated with and whose desintegration time was equal to 13,50 minutes.