Estudio del contenido de polifenoles, capacidad antioxidante y antiinflamtoria del extracto obtenido de tipo (Minthostachys mollis Griseb)

Abstract

The present research work is focused on the extraction of secondary metabolites, specifically phenolic compounds present in the tipo leaves (Minthostachys mollis Griseb) and the evaluation of their biological activities. Tipo plant extract was optimized by different factors (solvent, temperature, time and number of successive extractions) of these, methanol 50 percent, 50 Celsius degrees, 10 min with 4 successive extractions presented the optimal conditions to obtain the highest polyphenol and flavonoids content which was 70,115 more less 0.440 mg gallic acid equivalents per gram dry sample and 64.967 more less 0.858 mg catechin equivalents per gram dry sample, respectively. Two biological activities were evaluated, the antioxidant activity by the thiobarbituric acid reactive substances (TBARS) method where the highest percentage of inhibition 68.987 percent was obtained at 1000 ug per mL. The anti-inflammatory activity was evaluated by two methods: a) protein denaturation and b) human red blood cell membrane stabilization (HRBC) that presented an important percentage of protection (94.398 percent). Finally, the content of minerals present in tipo leaves was evaluated by atomic absorption spectrophotometry and a high content of Ca and Mg was obtained (13174.749 mg per kg dry base and 5771.208 mg per kg dry base, respectively). Due to the presence of these biological activities, tipo could become a natural alternative that substitutes synthetic products and contributes to the treatment of various pathologies, in addition to providing essential nutrients to the human being.