Producción de la enzima recombinante Cel6D de Paenibacillus barcinonensis para la degradación de residuos agroindustriales

Abstract

The present research study aims to obtain the cellulase enzyme from the expression of Paenibacillus Barcinonensis Cel6D protein in E. coli BL21 (DE3) and BL21 (TFH2) bacteria, and analyze whether the cellulase enzyme has the ability to degrade waste agroindustrial through the apparent specific activity (U per mg protein). For greater accessibility of the enzyme to cellulose, the residues were subjected to mechanical pretreatment, and particle size reduction to increase the surface area and reduce the degree of crystallinity. The specific apparent activity measured through the quantification of reducing sugars after enzymatic hydrolysis at a temperature of 25 Celsius degrees at incubation times of 30, 120 and 240 min; the cellulase showed activity on the PASC, cane bagasse, cocoa husk, corn stubble with a value of 0.0001; 0.0028; 0.0016 and 0.0011 (U per mg protein). But no enzymatic activity was observed when the substrate was rice bran. Moreover, the identification of products of the enzymatic hydrolysis of agroindustrial waste was tested, showing that the celobiohidrolase Cel6D is capable of hydrolyze the cacao shell, cane bagasse and corn stubble producing cellobiose and occasionally glucose.