Producción de biofilms a partir de diferentes matrices biodegradables y evaluación de su actividad antibacteriana

Abstract

Biodegradable polymers have few viscoelastic, rupture, barrier and antibacterial properties affecting their use in industry. The objective of this research is to produce films with different biodegradable matrices, to improve their properties and evaluate their antibacterial effect by impregnating metal nanoparticles. It was determined that films produced by Komagataeibacter xylinus with a volume of culture medium of 50 ml produced thick films, as in films obtained from filmogenic mixtures with the increase to 20 ml of the final mixture. Stress tests such as axial force and tension related to the strength of materials were performed when axial force is applied in opposite directions; obtaining 21-24 N of axial force and 1.9-3.8 por ciento of tension for the commercial grade glucose per carrageenan mixture being the most representative test. The antibacterial evaluation showed that only films impregnated with silver have an antibacterial effect against E. coli and S.aureus; giving inhibition halos of 1.2 - 1.4 cm in diameter for CB films and 1.3 - 2.5 cm in diameter for films of filmogenic solutions evaluated in E.coli. On the other hand, values between 1.33 - 2.01 cm in diameter were obtained for CB films and 1.09 - 1.29 cm in diameter for films of filmogenic solutions evaluated in S. aureus.