Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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Tema: search.filters.subject.RHIZOBIUM SPP.

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    Item type:Ítem,
    Caracterización de Rhizobium spp. a partir de chocho (Lupinus spp.) en cuatro localidades del cantón Latacunga
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03)
    Tovar Roman, Jenifer Odalys
    ;
    Pérez Salinas, Ruth Narcisa
    The contamination from synthetic nitrogen fertilizers has become a problem affecting the ecosystem worldwide due to excessive use. The search for biotechnological alternatives such as the study of microorganisms that contribute to nitrogen fixation has become more relevant. Rhizobium spp. is responsible for nitrogen fixation in legumes, such as Lupinus spp., bioprospection of this bacterium is fundamental to alleviate the pollution from synthetic fertilizers. This study focused on characterizing Rhizobium spp. from chocho (Lupinus spp.) in four locations in the Latacunga canton. 32 samples were collected from the locations and 64 strains were isolated, according to phenotypic characterization and biochemical tests including catalase, methyl red, citrate, among others, and an authentication test (YMA + PB) where it was determined that 100 percent of the isolates correspond to said genus. Its genetic variability was demonstrated by PCR Fingerprinting in which 32 of the isolates in composite samples (16) were analyzed, where 13 isolates present a genetic variability of 55 percent with heterogeneity, belonging to the 4 locations, 2 present a genetic similarity of 93 percent with homogeneity corresponding to locations 2 and 4 (Mulalo and Poalo), and a unique isolate from the Ignacio Flores location. Based on the results obtained, it can be said that the isolates can belong to the genus Rhizobium spp. with high genetic variation.
  • Miniatura
    Item type:Ítem,
    Caracterización de Rhizobium spp. a partir de haba (Vicia faba L.) en cuatro localidades del cantón Tisaleo
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03)
    Morales Miranda, Cynthia Monserrath
    ;
    Pérez Salinas, Ruth Narcisa
    Agricultural productivity depends on the nutrients available in the soil, especially nitrogen. Currently, chemical fertilizers are used to improve production, without considering the pollution they generate. Biotechnological alternatives give a new approach to nitrogen fixation in legumes. In this research, nodules from broad bean (V. faba) were used, a commercial and essential legume due to its high content of proteins, vitamins and minerals; capable of fixing nitrogen through Rhizobium spp. 32 samples were collected in 4 locations of Tisaleo that were morphologically characterized, where 90 percent of them presented characteristics similar to Rhizobium spp. Biochemical tests were performed where 100 percent of these were positive for methyl red, Voges Proskauer, starch hydrolysis, and catalase, however, 98 percent of these were negative for hydrogen sulfide and TSI. A BOX-PCR was developed, to identify the existing genetic distance between them, from composite samples (16), great genetic variability was evidenced, with the formation of 2 groups from a similarity coefficient of 51 percent, being the sample that presented the most similarity in relation to the other QLU56 belonging to locality 4 and the samples that had a 95 percent similarity coefficient were ATS 78 and ATS12, belonging to locality 2. The results obtained are important for future research that involve the elaboration of biofertilizers as a friendly alternative to the environment.