Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

Examinar

Filtros

2018 - 201912020 - 20211

Ajustes

Tema: search.filters.subject.GENÉTICA MOLECULAR

Resultados de la búsqueda

Mostrando 1 - 2 de 2
  • Miniatura
    Item type:Ítem,
    Modelamiento computacional de la enzima acetohidroxiácido sintasa (AHAS) de Mycobacterium tuberculosis
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2021-09)
    Jurado Barona, Evelyn Katherine
    ;
    García Solís, Mario Daniel
    Tuberculosis is a disease caused by the pathogen Mycobacterium tuberculosis. It annually produces millions of deaths worldwide. The severity of this condition is caused by the progressive appearance of multidrug resistant strains (MDR-TB) and extensively drug resistant tuberculosis (XDR-TB). For this reason, it is common to use long treatments that produce unwanted effects in patients. Given the lack of effective and well tolerated drugs to treat tuberculosis, it is necessary to investigate new targets which will slow down the spread of the pathogen. The enzyme acetohydroxyacid synthase (AHAS) is amongst the most promising targets. AHAS is the first enzyme in the branched chain amino acid biosynthetic pathway (BCAAs). It has been already shown that AHAS is crucial for the survival of Arabidopsis thaliana, Candida albicans, Saccharomyces cerevisiae, and other organisms. Here, a model of Mycobacterium tuberculosis AHAS complex was obtained using the Swiss-Model. The model was derived from its closest structure, S. cerevisiae AHAS. The MtAHAS complex exhibited the same structural features described previously for AtAHAS and ScAHAS, including its resemblance to the Maltese cross. The interactions with cofactors and the most important motifs involved in the inhibition with methyl metsulfuron were elucidated using the software WinCoot and LigPlot. The hexadecameric complex of MtAHAS generated provides a platform for the rational design of enzyme inhibitors for the effective treatment of different strains of Mycobacterium tuberculosis.
  • Miniatura
    Item type:Ítem,
    Derarrollo de herramientas de biología sintética para la bacteria no modelo Burkholderia sacchari: un nueva plataforma para una producción más limpia
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2018-11)
    Barreno Sánchez, María José
    ;
    Pérez Betancourt, Yunys
    The metabolic diversity in microorganisms can provide the basis to create new biochemical products. However, most metabolic engineering projects use a handful of established model organisms and, therefore, a challenge to take advantage of the potential of novel microbial functions is the ability to express new genes or directly use non-model organisms. Genetic manipulation of non-model microorganisms is still a challenge due to the specific nuances of the organism that make universal molecular genetic tools difficult. However, in recent years, unprecedented advances have been made in synthetic biology and the development of molecular genetic tools. That is why the objective of this study is to develop useful tools of synthetic biology to enhance the use of the non-model bacterium Burkholderia sacchari, as an alternative to biological production, since it has been proven that it can synthesize several high-value products using different sources of carbon. In this work we used the BglBrix plasmids, a standard that includes all the basic biological parts necessary for a vector to be efficient, stable and fulfill its function within the cell. The transformation of Burkholderia sacchari cells with the BglBrix plasmids was carried out by chemical method using RbCl, obtaining high transformation efficiency. Dose-induction assays were performed to evaluate the expression of fluorescent proteins at a maximum concentration of inducer.