Ciencia e Ingeniería en Alimentos y Biotecnología

URI permanente para esta comunidadhttp://repositorio.uta.edu.ec/handle/123456789/412

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2016 - 201922020 - 20251

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  • Miniatura
    Item type:Ítem,
    Bioprospección de microorganismos autóctonos productores de biosurfactantes aislados de suelos contaminados con aceites de lubricadoras en Ambato. Articulado al Proyecto de Investigación aprobado mediante Resolución Nro. UTA-CONIN-2023-0294-R
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02)
    Guerrero Carranza, Yadira Lizbeth
    ;
    Calero Cáceres, William Ricardo
    Hydrocarbon contamination represents a global issue due to its high toxicity and recalcitrance. This is mainly attributed to the indiscriminate use of oils of various types, ranging from edible oils to motor lubricants. In particular, motor oils have a significant impact on soil, as their heavy nature and low dispersion in water reduce the bioavailability of hydrocarbons for microorganisms. This phenomenon deteriorates soil structure over time. In this study, bacteria present in soils contaminated with oils from lubricant workshops in the city of Ambato were isolated, identified, and characterized. It is essential to highlight that biosurfactants, surfactant compounds of microbial origin, play a key role in this context. To identify biosurfactant-producing bacteria, various tests were performed, including the drop collapse test, oil spreading, hemolytic activity, emulsification, and the use of CTAB agar. Subsequently, biosurfactant-producing strains were identified using the MALDI-TOF technique to determine their genus and species. The results revealed that the bacteria belonged to Pseudomonas aeruginosa, known for producing rhamnolipids, a type of biosurfactant. Additionally, their antimicrobial activity was evaluated, and the phylogenetic similarity of these strains was investigated using the ERIC-PCR technique. Finally, an oil biodegradation study was conducted, comparing conditions with and without substrate enrichment to analyze process differences.
  • Miniatura
    Item type:Ítem,
    Evaluación de la capacidad de remoción de cadmio mediante levaduras (Metschnikowia pulcherrima, Lecythophora ps.LE034), aisladas del volcán Tungurahua.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2016-07)
    Aguas Bonilla, Daniela Magaly
    ;
    Dugarte Jiménez, Nahir Yerely
    This study determined the removal capacity of cadmium through the use of biomass of yeasts isolated from the Tungurahua volcano. It was chosed two species Metschnikowia pulcherrima, Lecythophora sp. LE034 and a third commercial species as reference Saccharomyces cerevisiae. The determination of cadmium in the removal process was tested by the modification of the colorimetric method of the dithizone by Ecuadorian technical standard INEN 0982. The number of colony forming units (cfu) and growth factor of a series of dilutions were determinated through McFarland curve and count per diffusion in plate. The percentage of inhibition of growth against cadmium using concentrations from 107 up to 1,07 µg/l, was between 85% and 95%, being the yeast Lecythophora sp. LE034 the most susceptible to inhibition. The removal process showed that the three yeasts are able to efficiently remove between 88-89% of cadmium.
  • Miniatura
    Item type:Ítem,
    Evaluación de la capacidad de remoción de cadmio mediante levaduras (Rhodotorula mucilaginosa y Cryptococcus gastricus), aisladas del volcán Tungurahua.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2016-06)
    Jácome Gavilánez, Marco Antonio
    ;
    Dugarte Jiménez, Nahir Yerely
    The removal capacity of cadmium by Rhodotorula mucilaginosa, Cryptococcus gastricus and Saccharomyces cerevisiae was determined by the colorimetric dithizone method. Cadmium removal by the yeasts was studied a pH of 6 and in an incubator orbital at a temperature of 27 ° C, and at concentrations of 34240 mg/l of cadmium, the removal rates were higher than 95 %, showing a high death rate of the yeasts in the removal process. In addition, the process of removing cadmium was tested at concentrations of 2140 mg/l, maintaining the conditions described above. In this case Rhodotorula mucilaginous showed a removal capacity of 100 % of heavy metal while C. gastricus and S. cerevisiae had a maximum capacity of 98,5 % and 99,5 % respectively.