Browsing by Author "Yugcha Yugcha, Michelle Estefanía"

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Determinación de un método eficiente para la germinación in vitro de semillas de Zapoteca aculeata
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2022-09) Yugcha Yugcha, Michelle Estefanía; Vargas López, José Homero
Endemic species are often among the most sensitive to environmental changes and disturbances and therefore most at risk of extinction. To solve this problem, in vitro seed germination is a suitable method to propagate threatened plant species for conservation. The present work aimed to determine an efficient method for in vitro germination of Zapoteca aculeata seeds. First, two disinfection treatments were applied to the seeds to calculate the percentage of contamination. Then, six pre-germination treatments were applied to the seeds to evaluate the percentage and time of germination. Finally, the seed germination process was characterised. At each stage, the treatment that gave the best germination percentage was chosen using analysis of variance to statistically verify the significant difference of the treatments and the significant differences between the evaluated groups were evaluated using Tukey's test. The best results were obtained with disinfection D1, reaching a germination percentage of 27.08 percent, this is due to the concentration and exposure time of the seeds in the disinfectant solutions. With the pre-germinative method T2 a germination percentage of 46.25 percent was obtained, and the seeds were the earliest to germinate, taking between 17.4 days. Therefore, it is recommended to use it in future research because there is no information in the scientific literature regarding research work on the germination of Z. aculeata seeds.