Browsing by Author "Villacis Grijalva, Joyce Elizabeth"

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    Análisis bioinformático de cuatro secuencias de genoma completo de Salmonella entérica de origen avícola
    (2020-03-19) Villacis Grijalva, Joyce Elizabeth; Calero Cáceres, William Ricardo
    The application of molecular epidemiological tools applied to microbiology provides valuable evidence to elucidate the origin of clinical outbreaks. Additionally, provides information that supports the opportune intervention and takes of control measures. Nowadays, the raising of new omics technologies, as new generation sequencing (NGS) of complete genomes and the development of bioinformatics tools are improving the knowledge and the understanding of the microbiology. The implementation of these technological tools in microbiological studies is allowing to study in real-time and low cost the evolution and dissemination of new pathogens that are circulating across our geographical area. Salmonella enterica represents one of the most important pathogens that deserves special attention, considering its implications in clinical and productive sectors. Considering these reasons, it is important the realization of epidemiological studies using bioinformatic tools that allow analyze the origin, dissemination, virulence mechanisms and resistance genotypes. In this study, four S. enterica serovar Infantis and Kentucky from layer poultry origin obtained in Tungurahua and Cotopaxi were analyzed through WGS and the bioinformatic tools of Center of Genomic Epidemiology (University of Denmark) and BaseSpace (Illumina Inc). All the strains were categorized as human pathogens. According to their sequence-type, it belongs to the ST32 (Infantis) and ST152 (Kentucky). The strains harbor a plethora of antibiotic resistance genes. It is worth noting the presence of the beta-lactamases blaCTX-M-148 and blaCTX-M-65, which show an emerging raising in clinical and animal origin isolates around the world. Finally, our sequences were compared with sequences of analogous bacteria from different origins and geographical locations through phylogenetic trees. Our sequences are related to clinical isolates from the United States, suggesting that their share a common ancestor.