Browsing by Author "Vaca López, Jéssica Leonor"

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    Evaluación de tres diluyentes naturales para semen fresco de conejo (oryctolagus cuniculus) en la inseminación artificial
    (2017-01-25) Vaca López, Jéssica Leonor; Rosero Peñaherrera, Marco
    The present investigation was carried out in order to evaluate the effectiveness of natural diluents used in the treatment of fresh rabbit semen (Oryctolagus cuniculus) in artificial insemination and its usefulness in the field of work. Three different treatments were used: coconut water, skim milk and aloe vera plus egg yolk (in amounts that were according to the volume of semen obtained for each dilution in a ratio of 1: 9), studying its effect on motility, concentration and sperm viability, as well as reproductive parameters (design rate and litter size) in artificially inseminated nulliparous rabbits. Motility and viability were evaluated at 30, 60, 90, 120 min after the dilutions were carried out, with coconut water being the best result, since at 120 min it showed less loss of its qualities compared to the other treatments performed in vitro, at the same time demonstrated a better performance in vivo improving the rate of conception and the size of the litter. Three mestizo male rabbits and 12 mestizo females were used, of which one male and four females were treated per treatment, nulliparous females with two previous positive jealousies, aged 6 months and weighing approximately 3.5 kg, and males with an age of 10 months and an average weight of 4.5 kg, distributed with a completely randomized block design (DBCA), with 3 treatments of 4 replicates and one animal per experimental unit. A single dose of 0.5 ml was used for artificial insemination with fresh rabbit semen added with the three types of diluents respectively, at a time of 30 min of the dilution. The study was carried out in the Atahualpa parish of the Canton Ambato, in an experimental enclosure of elevated mesh cages for a period of 45 days