Browsing by Author "Toasa Canseco, Ana Belén"

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    Efecto del ácido hipocloroso como alternativa terapéutica en el control de mastitis subclínica en vacas lactantes
    (2022-09) Toasa Canseco, Ana Belén; Lozada Salcedo, Efraín Euclides
    The objective of this research was to determine the effect of hypochlorous acid as a therapeutic alternative in the control of subclinical mastitis in lactating cows. The study was carried out at the "Santa Clara" farm, in a dairy herd of 340 Holstein lactating cows, a total of 150 animals were sampled, of which 36 were selected for the experiment. An experimental design based on a completely random statistical model (D.C.A) was used to evaluate the efficacy of 3 doses of hypochlorous acid (HClO): T1= 10 ml of HClO, 450 ppm, intramammary route, for 5 days; T2= 20 ml of HClO, 450 ppm, intramammary route, for 5 days and T3= 30 ml of HClO, 450 ppm, intramammary route, for 5 days) compared to a conventional or control treatment (T0= 10 ml of Cephalexin 200 mg + Kanamycin 100,000 IU, intramammary route for 3 days) in lactating cows that presented three different degrees of subclinical mastitis, with 3 repetitions for each treatment. The variables analyzed were: the somatic cell count (Ccs/ml) as an indication of inflammation, the Colony Forming Units count (CFU/ml) and the antimicrobial action of the applied treatments. When doses of 30 ml of HClO (T3) were administered intramammary, after the application of this product, a cell count of less than 200,000 Ccs/ml was recorded in the three degrees of subclinical mastitis. Three types of bacterial agents were identified: Staphylococcus aureus in 66%, Streptococcus spp. 24% and Escherichia coli 10%. In relation to the antimicrobial action of hypochlorous acid, a better response was obtained in the reduction of Staphylococcus aureus colonies with a dose of 30 ml, sharing levels of antimicrobial response of 100% in cows with grade I and II subclinical mastitis with a slight reduction in the control of S. aureus for grade III cows. For Streptococcus spp. the antimicrobial response was 100% in the three doses of HClO and with the conventional treatment in grade II and III cows. However, an isolated case was recorded where the T2 response decreased slightly (91.8%), which could be attributed to handling situations unrelated to the experiment. Finally, the bactericidal effectiveness in E. coli colonies was the same in all the applied treatments (100%).