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Browsing by Author "Solis Mayorga, Erick Xavier"

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    Evaluación de la actividad antioxidante y antiinflamatoria de las flores de Spartium junceum
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-03) Solis Mayorga, Erick Xavier; Pérez Salinas, Ruth Narcisa
    In rural areas of developing countries, traditional medicine is used as the only treatment for illnesses, since it is difficult for them to go to a hospital or because they cannot afford to buy the medicines. Traditional medicine has been found to have an endless number of benefits such as antivirals, antioxidants, anti-inflammatory, anticancer, etc. Therefore, the present research aimed to evaluate the antioxidant and anti-inflammatory activity of S. junceum flowers. Hydroalcoholic extracts were obtained by Soxhlet extraction and maceration with 96 percent and 70 percent ethanol, the extract by Soxhlet with 70 percent ethanol had the highest yield of 14.01 percent with a standard deviation of 3.64. The qualitative determination of secondary metabolites was developed by phytochemical assays and metabolites such as alkaloids, saponins, phenols, tannins, flavonoids and quinones were identified in different concentrations; and to verify the presence of these, infrared spectrophotometry was also performed. The antioxidant capacity was performed with the DPPH technique, where the Soxhlet extract with 70 percent ethanol obtained the highest antioxidant activity of 76.72 percent with a standard deviation of 0.01. Finally, the extracts were evaluated in the anti-inflammatory activity by inhibition of egg albumin denaturation, the highest inhibition of 76.55 percent with a standard deviation 0.05 corresponded to the Soxhlet extraction with 96 percent ethanol.

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