Browsing by Author "Ríos Villarroel, Rommel David"

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    Caracterización físico química, antibacteriana y antioxidante de propóleo de melipona ebúrnea de la región Amazónica
    (2017) Ríos Villarroel, Rommel David; Díaz Sjostrom, Pedro
    In the present investigation the antioxidant and antibacterial capacity of five samples of propolis of Melipona ebúrnea of the Amazonian region of Ecuador was evaluated. Obtaining these chimical results (phenols and flavonoids) samples M5 8.39 and 0.07, M4 4.21 and 0.03, M3 2.75 and 0.03, M2 4.14 and 0.20 and finally M1 3.47 y 0.07expressed in mg of quercetin and mg gallic acid respectively. The test chemical oxidation of ferric ions to ferrous yielded the following values M5 0.230, M4 0.104, M3 0.128, M2 0.075 and M1 0.361 expressed in mg per gram ferro ammonium sulfate crude propolis. The minimum inhibitory concentration by the microdilution method ethanolic extracts of propolis Melipona ebúrnea has good results bactericides, obtained in Salmonella enteritidis ATCC 13076 and Pseudomonas aeruginosa ATCC 25925 inhibition to the 10%, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC 33495 and Staphylococcus aureus ATCC 2592 to the 5% and Streptococcus agalactiae ATCC 13813 to the 7.5%. In the method of plate-disk for bacterial susceptibility strains Pseudomonas aeruginosa ATCC 25925 and Klebsiella pneumoniae ATCC 33495 have a total resistance, Salmonella enteritidis ATCC 13076 and Escherichia coli ATCC25922 partial sensitivity observed, while in Staphylococcus aureus ATCC 2592 to greater concentration better effects of sensitivity and finally Streptococcus agalactiae ATCC 13813 a partial resistance was observed in the evaluated concentrations. Showing a positive effect of propolis in enteropathogenic bacteria Escherichia coli and Salmonella enteritidis and total sensitivity Staphylococcus aureus.