Browsing by Author "Moya Castillo, Erika Vanessa"

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    Evaluación de la actividad antioxidante, antiinflamatoria y citotóxica in vitro de los extractos vegetales de Marco (Ambrosia arboresces) y Quishuar (Buddleja incana), obtenidos mediante secado por aspersión
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-07) Moya Castillo, Erika Vanessa; Pérez Betancourt, Yunys
    The tradicional use of Marco (Ambrosia arborescens) y Quishuar (Buddleja incana) for their medicinal properties has been the subject of research aimed at scientific advances. The watery, hydroalcoholic (50:50) and ethanolic extracts of Marco leaves were used in in vitro experimentation in order to determine antioxidant, antiinflammatory and cytotoxic biological activity. The concentration of antioxidant activity was determined using the chemiluminiscence method in which the best effective treatment was the hydroalcoholic extract (50:50) of leaves of Quishuar 180629,191 ±293,399 μg galic acid/g extract. The antiinflammatory activity was evaluated using the membrane stabilization method with human erythrocytes, in which the ethanolic extract of Marco leaves showed 88,57 percent of antiinflammatory capacity, in contrast to the antiinflammatory control using asprin, measured at 87,66 percent. The cytotoxic capacity was determined with a colorimetric MTT method 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide using MCF-7 cells. With the ethanolic extract, a cellular apoptosis was produced, obtaining an IC50 of 0.0043. This evidences that with a minimal quantity of the extract, lethal toxicity levels were reached in the cells. The results obtained confirm that the disolvent used in the extraction of secondary metabolites affects the biological activities of the plants. In addition, Marco and Quishuar, being plants native to the Sierra region, are exposed to extrinsic factors such as climate, temperature, wind and rainfall that affect the production of metabolites in plants.
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    Incremento de la solubilidad de la curcumina extraída de la Cúrcuma longa L con mecanismos físicos y mecánicos.
    (Universidad Técnica de Ambato. Facultad de Ingeniería en Sistemas, Electrónica e Industrial. Maestría en Química, 2021) Moya Castillo, Erika Vanessa; López Hernández, Orestes Darío
    The low availability of curcumin is a limiting factor to take advantage of its bioactive properties, anti-inflammatory, antibacterial, cytotoxic, hepatoprotective, antioxidant, among others. New alternatives to allow insoluble compounds in aqueous media to increase their solubility through a nanoemulsion are efficient proposals because nanoemulsions can rapidly transfer the active principles they possess, a characteristic that makes them ideal for industrial, pharmaceutical, biotechnological, and food applications. Obtaining curcumin from the rhizomes of Curcuma longa L. was possible with solid-liquid extraction, using ethanol as a solvent due to its chemical affinity with curcumin (liposoluble phytochemical compound). The solvent concentration, the ratio of plant matter to solvent volume (m/v), temperature, and contact time were the factors considered for the extraction. Based on the results of statistical analysis, were established as optimal conditions: ethanol concentration (95 %), m/v ratio (1:8), temperature (80 °C), and time (90 minutes), to optimize the extraction process and obtain an extract (EMC73) with 22.72 % curcumin. The nanoemulsion formulation consisted of a mixture of curcumin extract with Tween 80 and vegetable oil, proportions established according to the proposed experimental design, taking into account the constant agitation factor so that the components interact adequately. The treatment that presented the highest percentage of transmittance 87.58 % had a composition of 45 % extract-Tween 80 mixture, 35 % vegetable oil, and 20 % water, considering that as the transmittance of a sample increases, the size of the particles in suspension decreases. Tween 80 and Eumulgin CO 40 were used as non-ionic emulsifiers to guarantee the stability, solubility, and emulsifying effect of the nanoemulsion, considering the Hydrophilic-Lipophilic Balance (HLB). When measuring the absorbance of the samples to test the solubility of the nanoemulsion in aqueous medium, 0.14 absorbance was obtained, which is a direct relationship of the curcumin concentration of the nanoemulsion dissolved in aqueous medium