Browsing by Author "Apunte Benalcázar, Denise Ivone"

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    Obtención de un medio enriquecido en proteínas a partir de residuos de papa (Solanum tuberosum) por fermentación microbiana
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2019-08) Apunte Benalcázar, Denise Ivone; Fernández Rivero, Danae
    In the present work of investigation to obtain a half enriched in proteins by microbial fermentation, the physicochemical characterization of the domestic residues of potato was carried out (Solanum tuberosum), for this it was determined: pH, percentage of humidity, percentage of starch and concentration of protein in the superchola, chola and única varieties. By means of a completely random design it was possible to verify that there was no significant difference between the three potato varieties in terms of the percentage of starch. It was characterized macroscopically and microscopically to Aspergillus niger, microorganism isolated by students of the Faculty of Science and Engineering in Food and Biotechnology from organic substrates of lemon, to later use it in the microbial fermentation with the domestic waste characterized. In the research 4 media were formulated, by means of a Factorial design 22 the influence of the humidity factors (61 percent and 93 percent) and composition of the medium (without salts and salts) on the variable responses was determined: concentration of proteins and concentration of biomass, obtaining optimum values of 15,91 mg for every ml and 0,79 g for every ml respectively, in the medium formulated without salts and with a humidity of 61 percent. The kinetic parameters of the microorganism in the optimized medium were determined, obtaining as a result a specific growth speed u=0,016 h^(-1), a doubling time t_d=43,08 h and a yield Y_(x/s)=4,49 g of biomass/mg of substrate consumed.