Browsing by Author "Altamirano Chérrez, Edgar Ricardo"

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    Bioprospección de bacteriófagos líticos contra bacterias patógenas causantes de marchitez bacteriana en cultivos selectos de tomate riñón en Tungurahua. Articulado al proyecto de investigación aprobado mediante Resolución Nro. UTA-CONIN-2023-0294-R.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Altamirano Chérrez, Edgar Ricardo; Calero Cáceres William Ricardo
    Bacterial wilt is one of the most serious plant diseases worldwide, caused by the presence of pathogenic bacteria in soil and plants. It mainly affects the Solanaceae family, including tomato crops, causing large economic losses due to the destruction of crops. In this study, bacteria present in crops with bacterial wilt symptoms of tomato crops in Tungurahua were isolated, identified and characterized. Biochemical and morphological tests were performed to rule out strains of no interest in the study. A MALDI-ToF analysis was then performed in order to identify the pathogen strains causing the disease. K. cowanii and C. flaccumfaciens were found to be the main plant pathogenic bacteria in the area. Additionally, 20 bacteriophages present in soil samples with different types of morphology were isolated and purified, only against K. cowanii, due to the absence of bacteriophages for C. flaccumfaciens. By performing the drop test with different bacterial hosts, it was shown that the isolated bacteriophages have a high specific lytic capacity for K. cowanii and do not present any risk against beneficial bacteria. Bioprospecting of the bacteriophages found suggests their use as a potential biological control agent against important phytopathogenic diseases such as bacterial wilt. The results obtained highlight the use of bacteriophages as a sustainable alternative benefiting the agricultural systems of the province of Tungurahua.