Evaluación de bioaerosoles asociados en el sitio de desposición final de residuos sólidos en la Empresa Pública de Aseo y Gestión Ambiental del cantón Latacunga (EPAGAL)

Abstract

The bioaerosols present in the common waste area and in the hospital waste area of the final disposal site of solid wastes were evaluated in the Empresa Publica de Aseo y Gestion Ambiental of Latacunga canton (EPAGAL). Sampling was performed at five different points, recording environment temperature and relative humidity, for 24 days from Monday to Saturday at 10:00 a.m. and 2:00 p.m. in each of the zones, inoculating 1000 cc of air in breeding grounds Brain Heart Infusion. Microbial concentrations were estimated using a McFarland curve, with results in the range of 1,00135*1010 CFU/ml at 16 °C and 63% RH and 1,06*107 CFU/ml at 13 °C and 100% RH for common waste, while for hospital waste 6,9041*109 CFU / ml at 16 °C and 63% RH and 1,06*107 CFU/ml at 13 °C and 94% RH. By means of the IMVIC biochemical tests, a total of 91 bacteria were isolated, with six species of enterobacteria Shigella sonnei, Citrobacter freundii, Proteus mirabilis, Klebsiella pneumoniae, Citrobacter koseri and Escherichia coli and three genera Salmonella, Enterobacter and Providencia. It was not possible to identify 15 bacterial strains because they did not present bibliographically comparable results. The presence of enterobacteria and high microbial concentrations in these areas are the main cause of gastrointestinal diseases in both workers and surrounding populations.