Estudio del potencial antiinflamatorio y citóxico del extracto acuoso de hojas de diente de león (Taraxacum officinale)

Abstract

One of the approaches of the research is the development of drugs of natural origin for the treatment of pathologies. Plants, due to their great biodiversity and availability, are one of the main sources of active ingredients with pharmacological importance, the dandelion (Taraxacum officinale) is a weed that for its great content of active ingredients has been traditionally used for the cure of pathologies. In the present investigation, the capacity of the aqueous extract of dandelion leaves, obtained by spray drying, was studied as an anti-inflammatory and cytotoxic agent. The antiinflammatory activity was determined using the method of stabilization of the membrane of human erythrocytes, while the cytotoxic activity was evaluated using the method of bromide of 3- (4-5-dimethyldiazol-2-yl) -2,5-diphenyltetrazole (MTT ) with the breast cancer cell line MCF-7. The results obtained indicated that the aqueous extract of the leaves of dandelion presented anti-inflammatory activity from the concentration of 2.5 mg/ml and when using a concentration of 5.5 mg/ml presented an activity of 58.89 percet that exceeded to aspirin. In the case of cytotoxic activity, the IC50 obtained was 0.024, the extract showed a cell death percentage of 87 percent using a 1.00E-01 dilution starting from the 50 mg/ml stock solution. In conclusion, the aqueous extract of the dandelion leaves showed anti-inflammatory and cytotoxic activity when high concentrations of extract were used.