Caracterización biológica de andamios elaborados con nanocelulosa vegetal y quitosano. Articulado al proyecto de investigación aprobado mediante la Resolución Nro. UTA-CONIN-2025-0029-R

Abstract

This research focuses on the study of biological scaffolds, which are considered three-dimensional structures that mimic the support of the extracellular matrix, facilitating cell proliferation and adhesion. The main objective was the biological characterization of scaffolds made from plant-derived nanocellulose and chitosan through hemostatic assays. The methodology was experimental, since the hemostatic indices of the proposed scaffolds were previously unknown. Therefore, tests were conducted for the first time, varying the concentration of the polymer solution to determine the optimal formulation for scaffold development as a three-dimensional model for culturing prostate cancer cell lines. The study followed a mixed-methods approach: qualitatively, the scaffolds were evaluated in terms of shape, consistency, and size; quantitatively, six hemostatic tests were conducted—degradation, maximum swelling, coagulation index, hemolysis, red blood cell adhesion, and platelet adhesion. Results showed that scaffolds with higher chitosan concentrations exhibited better structural integrity and more efficient performance in the assays, whereas those with lower concentrations were fragile and displayed suboptimal ranges for use as 3D models in cell culture.