Evaluación de distintos medios de cultivo in vitro para la elaboración de micelio del hongo ostra blanco (Pleurotus ostreatus) (Jacq.) P. Kumm., en laboratorio

Abstract

The work was carried out in the microbiology laboratories of the Faculty of Agricultural Sciences of the Technical University of Ambato, located in the Cevallos canton, Tungurahua province, with the purpose of evaluating different in vitro culture media for the elaboration of mycelium of the fungus. white oyster (Pleurotus ostreatus) (Jacq.) P. Kumm., The culture media used were four that resulted in four repetitions for each culture medium composed of Agar with malt extract; culture medium composed of potato, agar, dextrose and yeast; culture medium composed of wheat extract, malt extract, wheat straw extract, agar and dextrose; culture medium composed of Agar - Agar. The completely randomized design with four repetitions was used, the data of the response variables were subjected to analysis of variance (ANOVA) and those variables that show significant differences will be subjected to the comparison of means test according to Tukey at 5%, in For the evaluation of the different media, four repetitions of each elaborated culture medium were carried out and it was possible to determine that in the Agar - Agar medium no differences were registered, since once the culture medium was made and seeded with a segment of the oyster fungus white (Pleurotus ostreatus) (Jacq.) P. Kumm., showed mycelial growth with an average of 2.75 cm in the following culture media: Agar culture medium with malt extract; culture medium composed of malt extract, wheat extract, wheat straw, agar and dextrose; Culture medium composed of Agar - Agar after 20 days of remaining in the incubator, while in other culture mediums composed of potato, Agar, dextrose and yeast, results were recorded with greater growth after 20 days, since it began to Grow the mycelium with an average of 4.93 cm in the culture medium composed of potato, agar, dextrose and yeast. Sowing was carried out in barley seeds with the help of polyethylene plastic bags where they remained in the incubator wrapped with aluminum foil for 20 days; once with the biomass in each bag, the mycelium was planted in bags with substrates made with barley seeds and with straw mince, where the growth of the basidiocarps was observed after 20 days.