Identificación rápida de Staphylococcus Aureus Meticilino Resistente, mediante la Técnica Slidex Mrsa en personal de salud portadores sanos del Hospital Básico Salcedo

Abstract

Introduction: Staphylococcus aureus is a Gram positive, nosocomial pathogen. MRSA is associated with resistance to multiple antibiotics, which makes its control and treatment difficult, being located in the nostrils of healthy people, in health personnel for being in direct contact with patients. Objective: The presence of methicillin-resistant Staphylococcus aureus was identified from cultures of nasal samples using the Slidex MRSA Technique from healthy carrier patients. Methodology: The present development project is quasi-experimental and crosssectional, since a study was carried out where the identification of MRSA is sought, using the Slidex mrsa technique. In which the percentage of patients who are healthy carriers of resistant microorganisms will be determined. Results: Of the 36 participants, 35 cultures were obtained with bacterial growth in Blood Agar, which represents 97.2%, of this percentage 97.1% were Gram positive arranged in a Staphtype cluster, while 2.9% were Gram negative. The work was only developed with gram positives which were planted in Mannitol Agar to continue with the study, of the 34 cultures planted 6 were mannitol positive representing 17.6%, of all the mannitol positive microorganisms 100% were coagulase positive and For the identification of Mrsa, the traditional procedure was used: sensitivity to Oxacillin by the disc diffusion method, to which 5 samples presented resistance, which represented 83% of the microorganisms that were coagulase positive. These same microorganisms were processed with the Slidex Mrsa Technique and we observed that 100% presented agglutination, allowing the detection of methicillin resistance. Conclusion: The Slidex MRSA rapid identification technique, when compared with traditional identification methods such as oxacillin resistance, allowed to achieve even better results and faster