Sistema bioelectrónico de detección de leucemia mieloide crónica en muestras de sangre, en el Laboratorio Clínico Ambalab de la ciudad de Ambato

Abstract

This degree project deals with the implementation of a Bioelectronic System for the detection of Chronic Myeloid Leukemia in blood samples, in the Ambalab clinical laboratory in the city of Ambato. Currently, this disease has no cure, mainly affecting asymptomatic people or people with low social status, which is why it generates a high mortality rate. The developed device is based on the analysis of blood samples applying the cyclic voltimetry method, acquiring information from 0.05mL of plasma centrifuged from the blood of patients, through the use of a biosensor formed by an electrochemical cell made with three nuclei of graphite electrodes. This result is sent to a database hosted on the Firebase platform through the Wifi Lora 32 microcontroller, which also allows monitoring the status of the device. Prior to the analysis of the acquired data, the number of cycles and the sweep speed must be configured, through the developed mobile application, considering the current variations of the working electrode in a certain voltage range, generating as a result the possible diagnosis. of LMC by hysteresis plot analysis. This data can be stored together with the patient's medical file, allowing the doctor in charge, in case he needs to review the information, to export a history in an Excel file, through the use of a mobile application and web interface.