Contaminación microbiana en el Hospital Docente Veterinario de la Universidad Técnica de Ambato

Abstract

The objective of this research was to identify the presence of nosocomial bacteria in the Veterinary Teaching Hospital of the Technical University of Ambato. 144 total samples were collected was performed which bacteriological culture and biochemical identification, samples were taken with sterile swabs with means of transport (Stuart), the hospitable environment isolates were performed by exposing agars blood during the night, the samples were taken twice per week during one month, on alternate days in specific areas: Clinic room 1 and 2, Hospitalization room 1 and 2, Operating room 1 and 2. Kirby-Bauer method was applied for the determination of sensitivity to desinfectants and antiseptics. The applied methodological design was a descriptive study with a design completely at random. For the processing of information, was conducted an analysis of variance and Tukey test at 5% for treatments that were significant through the program statistical Infostat version 2017. The obtained strains were: Staphylococcus aureus (21%), Staphylococcus coagulase negative (13%), Staphylococcus epidermidis (6%), Staphylococcus saprophyticus (0.67%), Gram positive bacilli (59%). The results of the effectiveness of disinfectants and antiseptics against found strains, showed greater effectiveness of the quaternary ammonium against S. aureus, S. coagulase negative; glutaraldehyde acted better for S. epidermidis and S. saprophyticus, was finally observed efficacy of chlorhexidine against gram-positive bacilli. The study shows the presence of potentially causing pathogens of nosocomial infections, in all the areas studied, the results can help to prevent the spread of these pathogens and improve the quality of the hospitable atmosphere of the Hospital veterinarian teaching of the Technical University of Ambato.