“Correlación entre la técnica de esteatocrito ácido y tinción sudan III en heces para el diagnóstico oportuno de esteatorrea en niños con desnutrición grado I”

Abstract

In the present investigation seeks to determine the presence of steatorrhoea by application of esteatocrito acid and Sudan III staining technique as timely and differential markers of fat in stool, stool samples of 100 children who had diarrhea were included. I was done in the basic Hospital los Andes of the Pillaro canton.

Two qualitative-quantitative methods were used for the analysis of the samples, the technique of acid esteatocrito 5 M perchloric acid fat was used is extracted by acidification and is separated from the rest of matter solid by centrifugation, measured the thickness of each layer and the esteatocrito is expressed by a percentage that is the layer of fat on the total of fecal matter. While for the analysis of Sudan III used dye said, and was observed by microscopy fat drops by field through the lens of 40 x.

The total of the population under study was determined that 21% of the children gave favorable results for steatorrhea with 21 of these cases were identified by the technique of acid esteatocrito and while 16 patients were identified by staining Sudan III also found that 5 positive cases were not detected by Sudan III. By test Chi square with the data obtained through the relationship between staining tests Sudan III and acid Esteatocrito is can I determine that it is significant, since the value of X2t = 3,841 < X2c = 71.655. As the X2c, calculated is greater that the X2t estimated in the table, the null hypothesis was rejected and to accept the hypothesis alternative that I mention that the technique of acid esteatocrito, is correlated with the staining Sudan III in the diagnosis of steatorrhea in children with malnutrition Grade I. By which it was determined that the test of choice to diagnose steatorrhoea is the technique of esteatocrito acid.