“Actividad citotóxica de extractos de uncaria tomentosa y mansoa alliacea en líneas celulares de cáncer de mama”

Abstract

Breast cancer is a disease that has claimed the lives of many women around the world, due to it is great impact on the population this disease represents a critical public health problem that requires more research at the therapeutic level. In Ecuador the use of ancestral medicine to relieve infectious diseases through plants with antitumor properties is a custom that has been preserved up to now. Given that our country has considerable botanical diversity, it increases the motivation to evaluate and corroborate the properties attributed to them. This research project aimed to determine the cytotoxic activity of ethanolic extracts of Uncaria tomentosa y Mansoa alliacea on breast cancer cell lines by means of techniques of culture and cell viability. Several studies describe that the extracts of these species generate a toxic effect on tumor cells, which makes them candidates to be studied chemically and pharmacologically with broad future perspectives. In this research the extracts of the species Uncaria tomentosa and Mansoa alliacea were obtained through maceration techniques with ethanol. The concentrates were lyophilized and resuspended in PBS (phosphate-buffered saline), likewise, the amount of proteins was quantified using Bradford technique. The results from the MTT cell viability assay were: for the extract of Uncaria tomentosa an IC50 of 0.0903µg/mL, while for Mansoa alliacea it was 0.2205µg/mL. Finally, an analysis of variance (ANOVA) was carried out in addition to a Tukey post-hoc analysis. Significance less than <0.05 was obtained, which is why the alternative hypothesis was accepted, that described: Uncaria tomentosa and Mansoa alliacea extracts have cytotoxic activity on the MCF-7 ATCC® HTB-22 ™ cell line.