Caracterización fenotípica de actinomicetes aislados de suelos contaminados con hidrocarburos

Abstract

Ninety-three strains of actinomycetes, isolated from soils contaminated with petroleum derivatives, from the Sacha field were used for the determination of phenotypic and metabolic diversity. Eighteen groups-species were identified, presenting a similarity greater than 90 percent, after performing numerical taxonomy of phenotypic data. Likewise, all crops were grown in Bushnell-Hass, enriched with gasoline, diesel and oil, as the only source of carbon and energy. It was determined that 89 percent of microorganisms can use different hydrocarbons in their metabolism and that only the representatives of two groups-species do not have this capacity. 92.2 percent of actinomycetes can use gasoline as a substrate, 83.9 percent diesel and 60 percent oil. The results show that this group of actinomycetes presents a great phenotypic and metabolic diversity. These characteristics could be studied to determine the potential use in in-situ bioremediation processes.