Determinación de Enterobacterias Mediante Coprocultivo y su Relación con Gastroenteritis no Parasitaria en Pacientes Adultos que Residen en el Cantón Pujilí - Cotopaxi

Abstract

The research project was conducted in the area of Microbiology play an important role when analyzing biological samples, which helps with the diagnosis of the origin of infectious diseases.

The objective of this project was to investigate the types of bacteria that are causing acute gastroenteritis bacterial disease (GEBA), it was done with stool samples collected from residents adult patients from Pujili Canton by a pure culture, on selective and specific media, with an incubation of 24 to 48 hours in which to get a better result was complemented by biochemical tests as TSI (triple sugar iron), SIM (sulfur, indo, mobility), urea and citrate, of this way to identify the gender and species of the bacteria and finally a reseeding was done in the Agar Mueller Hinton to verify the susceptibility testing with the use of sensitivity discs broad spectrum, for which a bacterial dilution was performed with the standard 0.5 of the MacFarland turbidity scale.

The result was a population of 450 patients between 20 and 65 years old who attend to the Clinical Laboratory, after inclusion and exclusion criteria, a sample of 80 patients women / men who have had symptoms of GEBA was obtained.

So in this research project it was concluded that, 44 samples correspond to 55% which had growth of Escherichia. coli, 24 samples are equivalent to 30% of Salmonella spp development, and finally 12 samples corresponding to 15% of growth of Klebsiella oxytoca, reaching 100% of total samples, investigating the predisposing factors for appearance of GEBA, it was concluded that most cases are due to food contamination , and lack of personal hygiene