“Determinación de las cepas y la resistencia microbiana, de aislamientos bacterianos de pacientes con infecciones postquirúrgicas de cesárea en el hospital general docente Ambato”

Abstract

This research Project had as aim to isolate, identify; and evaluate the microbial resistance of the bacterial species from patients who suffered post-surgical cesarean section infection at the Hospital General Docente Ambato. The research method applied was descriptive, and transversal, as investigation technique was used laboratory methods, field research, and documentary compilation. The samples comprised in this work were 31 women (100%) who suffered post-surgical cesarean section infection, the age of the patients included were between 16 to 40 years, all of them accomplish the inclusion and exclusion criteria. The sample was taken at the infection site, under aseptic procedures using a sterile swab, and transported to the laboratory using Stuart medium for further processing. The sample was cultured in Blood Agar and MacConkey Agar. As part of this study, grown were evidenced in 22 samples (71%) and not grown was detected in 9 samples. The species isolated were: Staphylococcus epidermidis (41%), Staphylococcus aureus (23%), Escherichia coli (18%), Pseudomonas aeruginosa (9%) and Enterobacter spp (9%). Antimicrobial resistance were determined in vitro, applying disc immunodiffusion method using Muller Hinton Agar, (Bauer Kirby), gram positive bacteria’s showed an increased resistance to trimethoprim-sulfamethozole, clindamycin and erythromycin. Gram negative bacteria showed resistance to ciprofloxacin, amoxicillin-clavulanic acid and cefoxitin. As conclusion, gram positive bacteria were the microorganism most frequently isolated in bacterial culture of post-surgical cesarean section infection.